"MA1-023 was used in immunocytochemistry to describe the creation of a human iPSC line HEL24.3." AuthorsTrokovic R,Weltner J,Otonkoski T 技术参数 实验方法和说明书下载 PBM Anti-Human CD45 -Purified 产品类型:流式抗体 规格:25ug,100
图1.使用抗-TRA-1-60磁珠,MS分选柱和MiniMACS™Separator从MEF培养的iPSC的培养物中分离多能(TRA-1-60 +)iPSC。 分离后,将细胞用抗-TRA-1-60-PE(#130-100-347)和抗-Feeder-APC进行荧光染色,并使用MACSQuant Analyzer进行流式细胞分析。 根据散射信号和碘化丙啶荧光,将细胞碎片和死细胞排除在分析之外。
'In the last decade, there is an increasing application of induced pluripotent stem cells (iPSCs) for disease modeling. The iPSC technology enables the study of patient-specific neuronal cell lines in vitro to evaluate dysfunction at the cellular level and identify the responsible genetic factors....
说明书 实验方案 问题与答案 技术支持 抗体检测数据 (4) 已发表图片 (6) Application Created with Sketch. 图:1/10 TRA-1-60 Antibody (MA1-023-D488X) in ICC/IF Imunofluorescent analysis of TRA-1-60 (green) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed ...
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after recons...
Imunofluorescent analysis of TRA-1-60 (red) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with DyLight 650...
Analysis of Transcriptional Variability in a Large Human iPSC Library Reveals Genetic and Non-genetic Determinants of Heterogeneity. Authors Carcamo-Orive I,Hoffman GE,Cundiff P,Beckmann ND,D'Souza SL,Knowles JW,Patel A,Papatsenko D,Abbasi F,Reaven GM,Whalen S,Lee P,Shahbazi M,Henrion MYR,Zh...
The Molecular Probes human pluripotent stem cell (PSC) live imaging kits are the only kits offering superior imaging for live human PSCs in one box. This TRA-1-60 Alexa Fluor 594 Conjugate Kit includes an optimally labeled primary antibody
Imunofluorescent analysis of TRA-1-60 (green) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with Dy...
Characterization and differentiation of iPSC colonies generated on xeno-free conditions. Colonies were stained for pluripotent markers: NANOG (b), SSEA4 (c), SOX2 (e), Tra1-60 (f), and phase ((a) and (d)). (B) These colonies were differentiated in vitro into embryoid bodies (EBs) com...