TPR domains mediate protein–protein interactions, and, in a few cases, the exact features of the target protein that are recognized, and the specificity of the interaction are well established (Box 3). However, in the majority of cases, either the target protein itself or the region of the...
to a structural region of a Hop protein (or structurally related protein) by non-covalent interaction with the main and/or side chains of aa that are components of the TPR domain; (4) peptides (Ia), having the spatial model of (I), that can bind to a chaperone protein (CP) but are...
Briefly, protein extracts were prepared from cells lysed with buffer containing 1% (v/v) Triton X-100, 0.1% (w/v) SDS, and 0.5% (w/v) sodium deoxycholate, separated by SDS/PAGE, and transferred to nitrocellulose filters. Quenched membranes were probed with antibodies and analyzed using ...
Region between residue 2300 and the C-terminus (residue 2349) of Human Translocated Promoter Region Protein. 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0.25 mg/mL 纯化类型 Antigen affinity chromatography 保存液 TBS, pH 7.0 to 8.0, with 0.1% BSA 内含物 0.09% sodium azid...
Region between residue 600 and 650 of Human Translocated Promoter Region Protein. 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0.20 mg/mL 纯化类型 Antigen affinity chromatography 保存液 TBS, pH 7.0 to 8.0, with 0.1% BSA 内含物 0.09% sodium azide ...
29 More recent crystallographic analyses of Schizosaccharomyces pombe Cut9 (Cdc16/Apc6) and the N-terminal region of Encephalitozoon cuniculi Cdc27 showed that both these TPR proteins homo-dimerize through a structurally conserved N-terminal dimerization domain comprising seven TPR motifs.[30], [31...
The nuclear pore complex (NPC) consists of a conserved set of ~30 different proteins, termed nucleoporins and serves as a gateway for the exchange of materials between the cytoplasm and nucleus. Tpr (translocated promoter region) is a component of NPC th
protein degradation and host defence against invading pathogens. The crystal structures of many TPR domain-containing proteins have been determined, showing TPR motifs as two anti-parallel α-helices packed in tandem arrays to form a structure with an amphipathic groove which can bind a target peptid...
By way of investigating such TPR-mediated self-associations we used a variety of biophysical techniques to characterize purified recombinant Sgt1, a TPR-containing protein found in all eukaryotes that is involved in a broad range of biological processes, including kinetochore assembly in humans and ...
to a structural region of a Hop protein (or structurally related protein) by non-covalent interaction with the main and/or side chains of aa that are components of the TPR domain; (4) peptides (Ia), having the spatial model of (I), that can bind to a chaperone protein (CP) but are...