查阅seurat的github,对tpm数据的说明是 If you have TPM data, you can simply manually log transform the gene expression matrix in the object@data slot before scaling the data. You have to replace your object@data slot with the desired gene expression matrix as follows: sce.all@data = log(x ...
Normalized gene expression units provide consistent and comparable measures that can be used for performing differential expression analysis, exploratory data analysis, and comparing and visualizing gene expression counts within and across samples. Advantages of normalized expression units, Normalized gene expre...
If you have TPM data, you can simply manually log transform the gene expression matrix in theobject@dataslot before scaling the data. You have to replace yourobject@dataslot with the desired gene expression matrix as follows:sce.all@data= log(x = norm + 1)) 在Seurat v5 版本之前,直接跳过...