The codon adaptation index was calculated using the CAI Calculator 2[7]. Construction of GFP clones To construct GFP expression vectors carrying the native hydrophilic C-terminal sequence (MDELYK, 6 aa, hy +0.35) with a native stop codon (TAA), we removed the LeuGlu (Xho I recognition ...
did not show consistent total protein expression levels as calculated by the codon adaptation index (CAI)[7] of the local and entire sequences (data not shown). Therefore, we concluded that the reduction in total MK6-GFP expression by the deleted control clone...
Osram Sylvania, “ColorCalculator User Guide”, downloaded on Oct. 19, 2015 from www.sylvania.com, 50pp. Philips Color Kinetics, “IntelliWhite LED Lighting Systems,” downloaded on May 28, 2014 from http://www.colorkinetics.com/ls/intelliwhite/, 2pp. Philips Color Kinetics, “Color-Cha...