Total RNA Sequencing Workflow Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted next-generation sequencing (NGS) technology, producing approximately 90% of global sequencing data.* In addition to high-quality data, Illumina offers integrated whole-transcriptome sequencing workflows...
RNA-sequencing was performed to obtain the rumen epithelial transcriptomes from 9 of the most efficient (low RFI) and 9 of the most inefficient (high RFI) animals. The bacteria and archaea 16S rRNA transcripts were identified using an in-house developed pipeline, enriched from filtered reads ...
RNA (RNA instability; cost and complexity of reverse transcription reaction; etc.), but these are not unlike standard RNA-sequencing for transcriptomics. The appropriateness of using RNA for the characterization of microbial communities has been debated as it is assumed that RNA content is linked ...
RNA-sequencing and found that these data were even more enriched with intergenic reads (6.8% versus 40%; Supplementary Fig.7c). Given the enrichment of intergenic reads, we applied TAR-scRNA-seq9, a gene-annotation-free pipeline that identifies transcriptionally active regions in single-cell RNA...
0.1 - 1 u… Input quantity See full details in the specifications table Learn Order Overview Applications Documentation Compare Figures Overview Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simple workflow with master-mixed reagents. ...
The advent of next-generation sequencing technologies and their application to RNA analysis [13] provides the opportunity for us to re-assess the relative mass of the nc-RNAs and, thus, directly pose the question of how prevalent and important this class of RNAs might be. However, care must...
the advantage of being very fast compared with the RSD, but the lack of an output of the paired amino acid distances removes this advantage compared to the RSD method. It would be very useful if the sonic paranoid pipeline included the resulting average amino acid distance of the ortholog ...
Sequencing resulted in 125,873,897 reads for immune-challenged and 118,142,837 reads for untreated animals. Sequence statistics of Illumina sequencing are listed in Table 1. The first C. floridanus genome annotation (v3.3) contains 17,064 protein-coding genes [19]. The updated version of the...
Amplicon sequencing of the fungal ITS region was performed on samples collected during F1 and F2. Since F3 exhibited low abundance of cultivable fungal counts, it was not possible to generate amplicons for further sequencing. Similar to what was done with bacterial sequences, OTU counts that wer...
Single-cell RNA sequencing studies have suggested that total mRNA content correlates with tumor phenotypes. Technical and analytical challenges, however, have so far impeded at-scale pan-cancer examination of total mRNA content. Here we present a method