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5b) and are enriched in housekeeping, essential50,51, cancer hallmark47 and transcriptional regulation pathway genes (RNA splicing and degradation and protein degradation; Extended Data Fig. 5c). As expected, selected genes also demonstrated increased chromatin accessibility52 versus non-selected genes ...
The aim of our work was to develop a low-cost, portable device for the fast and easy determination of total protein content by using PDMS-based lab-in-a-syringe technology with removal of 3D-printed channels. We proposed two designs with a one-step PDMS curing and a two-step PDMS-...
The SLN-all dataset processed with totalVI can be explored interactively with Vision at http://s133.cs.berkeley.edu:9000/Results.html. Public datasets were downloaded from 10X Genomics (PBMC5k: https://support.10xgenomics.com/single-cell-gene-expression/datasets/3.0.2/5k_pbmc_protein_v3; ...
(Amide II band) and 1400 cm− 1(symmetric stretching of COO- of amino and fatty acids). Amide bands confer information on the secondary structure of proteins and are sensitive to protein conformation by differing degrees [85]. The Amide I band is the most sensitive, and originates ...
In detection of protein analytes the most commonly used receptors are other soluble proteins or small molecules. This is despite the fact that ∼30% of the genome codes for membrane proteins and many of the receptors in a cell are membrane components. The limited use of membrane receptors suc...
Biological processes related to protein catabolism, amino acid transaminations, RNA splicing and blood coagulation were also found to be regulated by diet, while the expression of genes involved in protein and ATP synthesis differed between the half-sibfamilies. Conclusions Overall, the combined gene ...
Liao, Y. S., Benya, P. D. & McKellop, H. A. Effect of protein lubrication on the wear properties of materials for prosthetic joints.J. Biomed. Mater. Res.48, 465–473 (1999). ArticleCASPubMedGoogle Scholar Liao, Y. The effect of frictional heating and forced cooling on the serum...
The same authors documented an inverse correlation between hemolysis and levels of the erythrocyte membrane protein spectrin [79]. These results could support the hypothesis that structural alterations of erythrocyte membranes increase the susceptibility of these cells to hemolysis, leading to elevated ...
To verify our findings, we observed the nuclear translocation of the NF-κB p65 protein in RAW264.7 cells using immunofluorescence staining. The results indicated that after TFCO treatment, the nuclear p65 expression was significantly decreased compared to M. pneumoniae-challenged cells (Figure 6A). ...