GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of...
CST's PathScan® Total Akt1 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total Akt1 Sandwich ELISA Kit #7170. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (10
vimentin, phosphatidylinositol 3 kinase (PI3K), protein kinase B (AKT), B-cell lymphoma-2 (Bcl-2), Bcl-extra long (Bcl-xl), Bcl-2 associated death promoter (Bad) antibody, Alexa Fluor 594-labeled secondary antibody, the inverted microscope, the constant temperature carbon dioxide cell incubat...
327 Second, replacement of a genetically absent protein may stimulate the production of antibody to the missing component, thereby limiting the value of subsequent therapy. This consideration is of limited concern in persons with autosomally inherited disorders such as hereditary angioedema, whose serum...
AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). Activation of PI3K results in the generation of phosphatidylinositol (3,4,5)-triphosphate (PIP3). These lipid second messengers bind to the pleckstrin homology domain of AKT to...
After incubation with primary antibody (anti-PKCε, 1:100) overnight at 4 °C, cells were incubated with fluorescein isothiocyanate-conjugated secondary antibody for 2 h at 37 °C. Nuclei were stained with DAPI (Beyotime Institute of Biotechnology, Shanghai, China). The immunofluorescence images...
CST 5315S Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (PE Conjugate) 100µl ￥4,264 PI3K / Akt Signaling Monoclonal Antibody 4C CST 5316S Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (PE Conjugate) 100µl ￥4,264 Protein Translation Monoclonal Antibody...
Subsequently, membrane was washed (three times, 5 min), and incubated with secondary antibody (1:5000) at 37 °C for 30 min. The blots were visualized with ECL-Plus reagent (Santa Cruz, USA) and analyzed with Image pro plus (IPP 6.0, Media Cybernetics, MD, USA) software. β-...
cells. The relationship between the MCF7 and MDA-MB-231 cell lysate protein concentration and the absorbance at 450 nm using PathScan®Total HER3/ErbB3 Sandwich ELISA Kit #7888 is shown in the upper figure. The corresponding western blot using HER3/ErbB3 antibody is shown ...
In CIA rats, the levels of anti-CII antibody, IgA, IgG and IgM in serum enhanced, BAFF, BAFF-R, PI3K, p-Akt and mTOR were highly expressed. Pae obviously inhibited B cell proliferation and decreased the expressions of BAFF, BAFF-R, PI3K, p-Akt and mTOR. The therapeutic effects of ...