蓝猪耳(Toreniafournieri L.)肌动蛋白基因的 克隆及生物信息学分析 植物学专业硕士研究生魏琦超 指导教师高峰教授 摘要 蓝猪耳(ToreniafournieriL.)是一种重要的热带、亚热带观花植物,盛花期较长,且具备特殊 的裸露胚囊,是一种研究植物花器官发育和授粉受精过程的理想模式植物。本研究以蓝猪耳为试材, ...
The complete chloroplast genome of T. fournieri was sequenced and analyzed by Illumina sequencing in this study. The full length of the complete chloroplast genome is 153,938 bp, containing a pair of inverted repeat regions of 24,805 bp (IRa and IRb) separated by a large single copy region...
而根据对杂交个体运用荧光原位杂交技术(Fluorescence in situ hybridization,FISH)的实验结果,这两个物种的染色体虽然数量不同,但基因组的亲和性相当高 (Nuntha B, Kikuchi S, Taychasinpitak T, et al. High Genomic Affinity between Torenia baillonii and Torenia fournieri Revealed by Genome Analysis Using a ...
In brief, leaf fragments of in vitro–grown torenia plants (Torenia hybrida 5 T. fournieri 3 T. concolor cv. Summerwave Blue) were cocultured with Agrobacterium for 7 days at 22uC in the dark. Kanamycin-resistant shoots were Chemical analysis. A piece of torenia leaf (25–50 mg fresh ...
CRISPR/Cas9, Flavanone 3-hydroxylase, Flower color, Genome editing, Torenia fournieriBackgroundCRISPR/Cas9 technology is one of the most powerful and useful tools for genome editing in various living organisms. In higher plants, the system has been widely exploited not only for basic research, ...
Torenia (Torenia fournieri Lind.) is an important flowering plant for scientific research with ornamental values. The flanking sequence of T-DNA insertion site was successfully amplified using TAIL-PCR in torenia, and the length of the amplified products ranged about from 200 bp to 2 000 bp and...
Genome editingRNAiToreniaSimultaneous knockdown or knockout of Torenia fournieri PLENA (TfPLE) and FALINELLI (TfFAR) genes with RNAi or genome-editing technologies generated a multi-petal phenotype in torenia. The MADS-box gene AGAMOUS (AG) is well known to play important roles in the ...
Torenia fournieriFlowers of honey plants (Torenia) face various abiotic stressors, including rain, that can damage pollens and dilute nectar. Many Torenia species are thought to have evolved a modified corolla base termed the corolla neck to prevent raindrops from contacting the nectar. Although ...
Through genome annotation, we identified 133 genes, including 87 protein-coding genes, eight rRNA genes, and 38 tRNA genes. Phylogenetic analysis revealed a close relationship between T. violacea and T. fournieri. The research results provide basic genetic resources for the development of species ...