DNA Topoisomerases, Type IIDNA-Binding ProteinsAntimetabolites, AntineoplasticDeoxycytidineEnzyme InhibitorsDrug Administration ScheduleAntigens, NeoplasmThe pyrimidine antimetabolite Gemcitabine (G) (2',2'-difluorodeoxycytidine) is used against several malignancies G exerts its antitumour effect mainly by ...
Human topoisomerases comprise a family of six enzymes: two type IB (TOP1 and mitochondrial TOP1 (TOP1MT), two type IIA (TOP2A and TOP2B) and two type IA (TOP3A and TOP3B) topoisomerases. In this Review, we discuss their biochemistry and their roles in transcription, DNA replication and...
Table 1 Lapcin activity against human cell lines. Fig. 4: Topoisomerase inhibition by lapcin. aType I topoisomerase DNA relaxation assay (SC, supercoil plasmid)bType II topoisomerase DNA decatenation assay (KDNA, Kineotoplast DNA).cType I anddII topoisomerase inhibition plots.n = 3 inde...
In mammals,topoisomerases are classified into two types,type I topoisomerase(Top1) and type II topoisomerase(Top2),depending on the number of strands cut in one round of action. Top1 induces single-strand breaks in DNA,and Top2 induces double-strand breaks. In cells from vertebrate species,...
DNA Topoisomerase II Assay The type II DNA topoisomerase was assayed by the decatenation of kDNA as described (19). The standard decatenation assay mixture (25 L), containing 25 mM Tris HCl pH 7.9, 10 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 50 mM NaCl, 10% glycerol, and 1 g ...
Briefly, type II enzymes (in blue) (1) bind two DNA segments [the gate segment (in green), which will be cleaved by the enzyme, and the transport segment (in yellow), which will be passed through the open DNA gate]; (2) bend the gate segment; (3) create a double-stranded break...
Mammalian topoisomerases are divided into two classes based on their structure and catalytic mechanism: Type I, such as Topoisomerase 1 (Top1), cut one strand of the DNA, whereas Type II, such as Topoisomerase 2 (Top2), cuts both DNA strands. Top2 enzymes introduce a double strand break ...
- BioMed Research International,2016,(2016-2-15) 被引量: 16发表: 2016年 Human topoisomerases and their roles in genome stability and organization Human topoisomerases comprise a family of six enzymes: two type IB (TOP1 and mitochondrial TOP1 (TOP1MT), two type IIA (TOP2A and TOP2B) and...
Unlike the type I form, topoisomerase II enzymes are found in multimeric form which are called a and b domains. These enzymes bind to the double strand of the DNA, forming double strand breaks in the DNA to pass through between two strands during replication steps. When the topoisomerase II...
Two kinds of DNA topoisomerases are recognized generally. Type I and type II enzymes catalyze topological changes in DNA by transiently breaking one stand or two strands of the DNA helix, respectively. The relaxation of superhelical DNA is a characteristic reaction catalyzed by a topoisomerase I ...