TOPO TA Cloning Kit TA Cloning Kit - Dual Promoter 常见问题解答 (FAQ) This may be caused by the instability of the insert DNA in TOP10 E. Coli. In this case, E.coli strains such as Stbl2, Stbl3, or Stbl4 have been shown to support the propagation of DNA with multiple repeats, re...
体外转录 TOPO TA Cloning Kit Dual Promoter TA 使克隆效率达到 95% 具有T7 和 SP6 两个适于体外转录的引物结合位点 Mach1™-T1R K461020 立即订购 TOP10F’ K460001 立即订购 TOP10 Electrocomp™ K466001 立即订购 MAX Efficiency DH5α™-T1 抗噬菌体 K462001 立即订购 测序 TOPO...
亚克隆用 TOPO™ TA Cloning ™ 试剂盒可提供高效的一步法5分钟克隆策略(“TOPO™ 克隆”),用于将 Taq 聚合酶扩增 PCR 产物直接插入到一个质粒载体上以进行亚克隆。每个试剂盒使用含便利限制性位点的 pCR™ 2.1-TOPO™ TA 载体进行亚克隆。根据您的需要和预算,您可选择含多种感受态细胞或不含感受态细...
TOPO® TA Cloning® Kits contain the popular pCR™2.1 TOPO® TA vector. TOPO® TA Cloning® Kits with Dual Promoter contains pCR™II TOPO® TA vector that allows subsequent in-vitro transcription following cloning. 技术参数
TOPO© 快速克隆技术介绍(1)|topo©Topo TA克隆方法(Topo TA Cloning Kit) Topo TA克隆原理与TA克隆一样,唯一不同的是TA克隆用的是T4连接酶把PCR片断连接到T载体上,而Topo TA Cloning用的是DNA Topoisomerase
Kit Options: TOPO® TA Cloning® Dual Promoter KitsTOPO® TA Cloning® Dual Promoter kits can be purchased with a variety of competent cells that deliver different advantages depending upon your needs:• General cloning: TOP10 cells (Cat. Nos. K4600-J10, K4600-01, K4600-40)• ...
Promoter: Polyhedrin Protein Tag: His Tag (6x) 储存 Bac-to-Bac C-His TOPO Expression Kit:• Vector kit for 20 TOPO cloning reactions, store at -20°C –pFastBac/CT-TOPO vector containing the C-terminal TEV cleavage sit and His-tag –pFastBac 1-Gus control plasmid (control expressio...
TRAM2 was PCR amplified from a human cDNA library and then inserted into the SP64 cassette using restriction cloning. Subsequently, the coding sequence of the TRAM2 mRNA was PCR amplified and inserted a parent pcDNA5-GFP-P2A-RFP cassette by Gibson Assembly. Flow cytometry analysis Analysis of...
体外转录 TOPO TA Cloning Kit Dual Promoter TA 使克隆效率达到 95% 具有T7 和 SP6 两个适于体外转录的引物结合位点 Mach1™-T1R K461020 立即订购 TOP10F’ K460001 立即订购 TOP10 Electrocomp™ K466001 立即订购 MAX Efficiency DH5α™-T1 抗噬菌体 K462001 立即订购 测序 TOPO...
ProductTOPO cloningAdvantagesOne Shot Competent cellsOrder now TOPO TA Cloning Kit Dual Promoter TA Get your clone with 95% efficiency Dual T7 and SP6 priming sites for in vitro transcription Mach1-T1R K461020 TOP10F' K460001 TOP10 Electrocomp K466001 MAX Efficiency DH5α -...