In its native context, Cas3 nicks and degrades the non-target strand via its nuclease domain (Fig.1a). Because DNA degradation could be detrimental to the cell, we investigated the requirement of the nuclease activity for the proper functioning of the CoMuTER system. To this end, we assesse...
insert. Further, the surface roughness can be compared with the tool wear of different grades of CBN–I, CBN-II& CBN-III inserts as shown in Figure 4a–c, respectively. It can be observed from the given Figure 4b that, the CBN-II inserts wear out due to the erosion of the CBN ...