The mean number of neurons was calculated for each animal and used for statistical analysis. Free-floating sections (30 μm thick) were incubated with GFAP (mouse monoclonal antibody, Immunological Science, Rome, Italy; dilution 1:1,000) or CD11b (mouse monoclonal antibody OX 42; AbD Serotec...
Nuclease free water was used in place of DNA in triplicate as the non-template control. Abso- lute gene transcription was quantified by averaging triplicate quantities of each sample for all test genes, following normalisation of the expression ratio of each using t...
gondii that seem to depend largely on activation of MyD88 for resistance30,47–49. The beneficial effect of the TLR-based interven- tions discussed here are at present largely hypotheti- cal, and their potential as therapeutic agents needs to be further tested in experimental models and in ...