split‐beta‐lactamasecomplementationhomogeneous assayphotoconjugationaffibodynitrocefinProtein fragment complementation assays (PCA) rely on a proximity-driven reconstitution of a split reporter protein activity, typically via interaction between bait and prey units separately fused to the reporter protein halves...
The resistant category was further equally split to denote ‘partially resistant’ and ‘strongly resistant’ subgroups. For patients treated with a CDK4/6i, those predicted to be sensitive had significantly longer survival than those predicted to be strongly resistant (log-rank P = 0.02, ...
Epitope scaffolds were multimerized on mi03 nanoparticles (NPs) using the SpyCatcher/Spytag23conjugation system in order to improve immune presentation (Fig.6a). Homotypic nanoparticles were developed that displayed 60 copies of designs Ex15, Ex19, or Ex20 respectively. A “mosaic” NP was also ...
This article discusses an expansion of cognitive load theory from individual learning to collaborative learning. As such, it attempts to help solve the conundrum of why collaborative learning in general, and computer-supported collaborative learning (CSCL) specifically, sometimes works while at other tim...
Finnish verb conjugation is very complicated. In Tampio, only some of the forms are used. In the table below, the verbs lisätä (to add) and tulostaa (to print) are conjugated in the relevant forms. FormTenseVoicePersonNumberSuffixlisätätulostaa Imperative Present Active 2 singular ...
As above, the negative-helicity result can by obtained through parity conjugation. FormalPara Exercise 2.3: A Amplitude from Collinear and Soft Limits Let us consider the collinear limit of the quark-gluon amplitude . We parametrise the limit with where . The collinear factorisation theorem impli...
4A). We first assessed the ability of SpyTag/SpyCatcher conjugation technology to enhance the fluorescence intensity of Split-GFP. The expression of the reporter gene Thy1.1 was used to correlate the intensity of GFP fluorescence with different expression levels. The conjugation technology significantly...
Specifically, S=R =R[d]/(d 2 ) is the ring of dual numbers of R, the conjugation β is the antiautomorphism of S given by β(r+r ' d)=α(r)-α(r ' )d, and the unit of symmetry v=u(1+d)· This makes it possible to regard split quadratic algebraic K-theory of R as ...
(* denotes complex conjugation) split by roughlyΩc(as seen in Fig.4), whereΩpandΩcare the Rabi frequencies of the probe and coupling fields, respectively. The dark state\(\left\vert D\right\rangle ={{{\varOmega }}}_{\mathrm{c}}^{* }\left\vert {5}^{2}{{{S}}}_{1/2}\ri...
Here, we describe a split-and-click convergent chemical route to individual or pools of sgRNAs. The synthetic burden is reduced by splitting the sgRNA into a variable DNA/genome-targeting 20-mer, produced on-demand and in high purity, and a fixed Cas9-binding chemically-modified 79-mer, ...