These anti-mouse IgG1 antibodies would then be further purified by passage through a chromatography column(s) containing mouse IgG2a, IgG2b, IgG3, IgM, etc., to remove any antibodies that cross-react with non-IgG1 isotypes. Additionally, secondary ...
Once unhinged from Keap1, Nrf2 is translocated into the nucleus where it has the capacity to heterodimerize with small MAF proteins and bind to Cis-acting AREs, effectively activating transcription of phase II detoxifying enzymes (see Fig. 1). Download: Download full-size image Fig. 1. Nrf2 ...
As noted above, lipid peroxidation requires both iron-dependent enzymes and iron-mediated Fenton reactions, thereby at least partly providing the iron-dependent nature of ferroptosis. Thus, interventions targeting iron metabolism have an impact on the vulnerability to ferroptosis. Ferric iron is the pri...
- The primary antibody does not recognize the protein in the species being tested: 1) Evaluate primary antibodies by dot-blotting first to how well they react with your protein. 2) Check the immunogen sequence, if provided, and determine if it is found in your protein. 3) If no immunogen...
much faster than uncatalyzed reactions Enzyme catalyzed reaction Reaction Rate = D[Product] D(time) [Product] Uncatalyzed reaction 0 0 Time Enzyme catalyzed reactions display saturation kinetics with respect to reactant concentration Important things to remember about enzymes just like other catalysts ...
Our approach relies upon the expression and purification of the non-phosphorylated phosphosite libraries and an active kinase of interest. The kinase is then reacted with the phosphosite library in vitro, enabling a proteome-wide phosphosite substrate screen (Fig. 4a). Reactions are subsequently ...
(see Methods - condensate preparation). Because this condensation is driven by non-specific depletion interactions23, additional proteins, such as enzymes, readily partition into the BSA-rich phase19. When the substrate of a partitioned enzyme is available in the dilute phase, these artificial ...
D Analysis of the binding ability of eight nanobody-HRP fusion proteins against PRRSV-1 and -2-N proteins by ELISA. E Comparisons of positive pig sera for PRRSV blocking five nanobody HRP fusion proteins to react with PRRSV-1-N protein. F Positive pig sera for PRRSV blocking six nanobody...
The mechanism of some enzymes makes it difficult to obtain a continuous optical change during reaction with an enzyme substrate. However, a discontinuous assay can often be developed by derivatizing the reaction products with one of the reagents described in Flu...
Iron–sulphur cluster-containing proteins include enzymes that contribute to maintaining genomic stability, as well as respiratory function. Iron regulates crucial signalling pathways in tumours, including the hypoxia-inducible factor (HIF) and WNT pathways. Measuring the expression of genes encoding ...