baumannii), the hyper Vi capsule variation in Salmonella strains is not associated with mucoidy (Supplementary Table 2). TviE function is altered by clinical missense mutations To determine the underlying molecular mechanism of how a single amino acid sequence variation leads to the variant form of...
A TCSPCS is used to generate a time trace of multiple detection events (mDEs), the histogram of which is matched to a probability model to determine the number of emitters. e, TIRF image of a U2-OS cell transiently transfected with the ProDOL probe. The arrows depict single-molecule ...
J Nanobiotechnol (2020) 18:20 https://doi.org/10.1186/s12951-020-0573-0 Journal of Nanobiotechnology RESEARCH Open Access Gold nanoparticle based double‑labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small ...
Peroxidase activity was detected using luminol chemiluminescent substrate from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). 3. Results 3.1. Strategy for Linking Biological Activities to Individual Tissue Fractions The overall strategy is shown in Figure 1. We used ion-exchange chromatography (IEC...
In short, putative chemoattractants, UV light-killed bacteria were added to the lower chambers and 30,000 eosinophils resuspended in KRG were added on top of the filters and incubated for 90 min at 37 °C. To determine the number of transmigrated cells, the microplate containing the lower ...
The IC50 determined according to WST-1 assay were utilized to determine the dose to be employed in the transcriptomics study, namely at ¼ IC50, a subtoxic doses that allowed to study the primary responses directly related to the exposure to these CNT, not the secondary ones and in order...
This raised the question whether immunity to subsequent infections by heterologous strains is affected by the first infection, a phenomenon known as the original antigenic sin [1, 32, 58]. We wanted to determine if initial infection with one variant affects immune protection to subsequent infection...
To determine the impact of ALDH2*2 mutation in ethanol-induced molecular changes in the brain, we treated wildtype (WT, ALDH2*1/*1) and ALDH2*2/*2 (homozygotic) mice daily with ethanol (1 g/kg/day) for 11 weeks, starting at the age of 9 weeks (Fig. 3a) and assessed ...
human neutrophils. Human neutrophils (2 × 105cells) were incubated without or with 450 μM Alb, then stimulated with 1 μM fMLF. ROS was measured using luminol (relative light units, RLU). ROS production from unstimulated neutrophils is negligible (white circles). Alb (gray circles...
In another aspect, the invention provides methods, kits, apparatuses, and systems, for screening a population of individuals, comprising assaying blood of an individual. In another aspect, the invention provides methods, kits, apparatuses, and systems for diagnosing an individual to determine whether ...