baumannii), the hyper Vi capsule variation in Salmonella strains is not associated with mucoidy (Supplementary Table 2). TviE function is altered by clinical missense mutations To determine the underlying molecular mechanism of how a single amino acid sequence variation leads to the variant form of...
Luminol-enhanced chemiluminescence (LECL) was used to determine the effect of soluble CD14 (sCD14) on the endotoxin inducible generation of reactive oxygen species in human monocytes. LPS is unable to activate monocytes under serum free conditions, but LECL responses were measured after pretreatment...
In BLMs, it was shown that the above cations induce high H+ conductance provided a fatty acid is added [Severina, I. I. et al., in preparation]. Most probably, we deal here with the same phenomenon as was previously described for mitochondria treated with tetraphenylphosphonium which was ...
NCF4, in contrast to NCF1, is known to preferentially activate the NOX2 complexes at endosomal membranes rather than at cellular membranes, and our previous results showed that the presence of WT NCF4 leads to a higher intracellular ROS response in neutrophils [11]. To determine the role of...
human neutrophils. Human neutrophils (2 × 105cells) were incubated without or with 450 μM Alb, then stimulated with 1 μM fMLF. ROS was measured using luminol (relative light units, RLU). ROS production from unstimulated neutrophils is negligible (white circles). Alb (gray circles...
ALDH2*2 deficiency increases damage by sustained ethanol exposure in mouse brains To determine the impact of ALDH2*2 mutation in ethanol-induced molecular changes in the brain, we treated wildtype (WT, ALDH2*1/*1) and ALDH2*2/*2 (homozygotic) mice daily with ethanol (1 g/kg/day) ...
For SPR analyses it is generally accepted to use monomeric and monovalent analytes in solution to determine the antibody binding kinetics according to a Langmuir model. Furthermore, it is rather advantageous for SPR measurements to use an analyte with higher molecular weight to increase the ...
iStock/LumiNola Could your dizziness be a sign of a mental health condition? Learn more with an online therapist There are several approaches to therapy that may help alleviate dizziness and identify the root cause of the issue. It can be important for individuals experiencing dizziness to seek...
For in vivo mouse study, two-way ANOVA followed by the Bonferroni post hoc test was used to determine the differences of two factors (i.e., RORA deficiency and maternal diabetes) in Mouse Protocol 1, and one-way ANOVA was used for analysis in Mouse Protocol 2. For the human study, ...
hnRNPH1 is required for embryonic development and male fertility To further determine the physiological roles of hnRNPH1 in the reproductive system, we first generatedHnrnph1global knockout mouse line (herein called hnRNPH1 gKO) by crossing germlineCredeleter mice withHnrnph1-floxed mice (see the de...