For standardized analyses, we used the titerplane technique as described in Niedrig et al. 200858 and the automated washing system MERGITE! (Euroimmun AG, Lübeck, Germany). serial two-fold dilutions from 1:10 to 1:5120 were used to determine antibody titers. For antigen-specific IgM ...
Mice immunized with these OVA-conjugated NPs showed enhanced CD8+ and CD4+ T cell response and a greater humoral response with balanced IgG1/IgG2 antibody titer indicating the accessibility of the antigen to both MHC class I and MHC class II processing pathways. To supplement the current ...
A presumptive case is a person with signs and symptoms that are clinically compatible with the disease under consideration, and for which there is laboratory evidence of infection (e.g., an elevated antibody titer but less than a fourfold increase), but the etiologic agent was not found in sp...
To determine whether miR-760 or HBEGF plays a role in OA progression in vivo, adeno-associated virus (AAV, virus titer 1.3 × 1012vg/mL, catalogue number: GC20200717HZCY-AAV01) was obtained from Hanheng (Hanheng Biotechnology Co., Ltd., Shanghai, China) to overexpress miR-760 ...
antibody response. The ZIKV type-specific neutralizing antibody response was defined as the post-DENV depletion neutralization titer divided by the neutralization titer in control-depleted serum. The difference between the type-specific response and the total response is the ZIKV and DENV cross-reactive...
Biopharmaceuticals have revolutionized the field of medicine in the types of active ingredient molecules and treatable indications. Adoption of Quality by
As ELISA measurements provide a more quantitative approach than histology, we used ELISA to measure soluble and insoluble Aβ, tau, and phospho-tau species to determine the effectiveness of the AV-1959R/A, AV-1980R/A, and combined vaccination approach. However, we also asked whether an immuno...
Similar work is therefore required to determine the level of co-infections in the GI tract, especially as this might impact on the severity of infection by SARS-CoV-2. The quantities of SARS-CoV-2 RNA in feces are also within the range reported for other respiratory viruses such as ...
MA). IgG from pooled samples that were collected from the macaques before immunization was purified as the control for passive antibody transfer. To determine ELISA titers achieved in mice by transfer of macaque sera, 200 μl of macaque sera with a reciprocal ELISA titer of 512,000 was inocul...
while all PS-containing ones displayed strong emission intensity. Lyso-PS nanoparticles exhibited the highest change in fluorescence intensity compared to double-chain PS, suggesting a much higher distribution of Lyso-PS on the outer leaflet of these vesicles. To determine whether particle size contribu...