本论文通过证明TNFα/CHX诱导的细胞凋亡主要依赖于Bak,而非Bax,首先阐明了Bak在TNFα/CHX诱导细胞凋亡中的作用,再通过不同抑制剂加入检测重要激酶活性,验证了p38,AKT,ERK及JNK在TNFα/CHX激发的信号传导通路途径中的相互关联,最后IP实验证明了Bak与AKT相互作用,TNFα/CHX处理后Bak-AKT复合物解离,Bak与AKT分别发挥...
We studied the effect of the knock down of the expression of Mtx1 and Mtx2 by interference RNA (shRNA) as previously described [6] on the subcellular localization of Bak in resting and TNFα/CHX treated primary cultures from Bax deficient glioblastoma multiforme patients (BdGBM) [6]. We ...
TNF activates a number of different death mechanisms depending of the cell type and circumstances. In many cell types, including mouse embryonic fibroblasts (MEFs), cell death triggered by TNF (plus CHX), or by related ligands such as TRAIL or FasL, requires the presence of the adaptor protein...
Indeed, as previously reported, RIP1-independent (TRADD- dependent) and RIP1-dependent apoptotic pathways can be activated when cells are stimulated with TNFα and CHX or TNFα and SMs, respectively.17 We found that NIK+/+ and NIK − / − MEFs equally died following exposure to TNFα/...
TNFR1 can trigger two independent apoptotic pathways depending on the cellular context and the stimuli. Indeed, as previously reported, RIP1-independent (TRADD-dependent) and RIP1-dependent apoptotic pathways can be activated when cells are stimulated with TNFαand CHX or TNFαand SMs, respectively...
Knockdown Gγ10 also partially inhibited TNF plus cycloheximide (CHX) plus zVAD-indued MEF necroptosis (Supplementary infor- mation, Figure S1D). Thus, Gγ10 participates in RIP3- dependent necroptosis in different cell types. Cell Research | Vol 24 No 4 | April 2014 Lisheng Li et al. ...
This is not the result of the disruption of the necroptotic cell death by Bad-deficiency, since non-cytotoxic dose TNFα was able to induce necroptotic cell death (Supplementary information, Figure S1f, Right panel), as previously reported,49, 50 in the presence of cycloheximide (CHX) and...