在光学显微镜下检查染色部分。 处理获得人体组织样本的另一部分,使蛋白质解结酶通过西斑检测βSMA(有关西方斑点分析的详细信息,见第7节)。 使用提取试剂(材料表)从人体组织样本的最后部分获取RNA,并通过RT-PCR将mRNA转化为cDNA。 通过qPCR 分析纤维化和细胞因子基因(有关 mRNA 制备的详细信息,请参阅第 6 节)。
Introduction Protocol Results Discussion Disclosures Acknowledgements Materials References 日本語 Automatically Generated Innis, S., Jacobson, K.Dietary lipids in early development and intestinal inflammatory disease.Nutr. Rev.65, S188-S193 (2007). ...
We aimed to 1) set up a TNBS-colitis protocol resulting in an endoscopic and histologic picture resembling IBD, 2) study the correlation between endoscopic, histologic and gene expression alterations at different time points after colitis induction, and 3) compare rat and human IBD mucosal ...
In conclusion, 4-methylesculetin showed similar efficacy to that obtained with either prednisolone or sulphasalazine, both in the acute phase of colitis as well as following a curative protocol. The intestinal anti-inflammatory activity by 4-methylesculetin is likely related to its ability in ...
Experimental design and treatment protocol2.4. Induction of colitis2.5. Tissue collection and preparation2.6. Measured parameters2.6.1. Change in body weight and colon weight/length ratio2.6.2. Colon macroscopic damage and area of colonic lesions2.6.3. Histopathological examination2.6.4. Biochemical ...
The current protocol was developed based on the ARRIVE guidelines for reporting animal research [23] and was approved by the Research Committee of the Escuela Nacional de Ciencias Biológicas (CEI-ENCB) (ENCB/CEI/002/2022). The animals were handled following the Mexican Federal Regulations for ...