Aside from skin cancer, breast cancer is the most common cancer among women, and it is the second leading cause of cancer-related death among women in the United States1. Approximately 15% of all breast cancers are triple-negative breast cancer (TNBC), which is estrogen receptor (ER)-negati...
incubated for 20 minutes at RT and in the dark. Finally, cells were fixed in 1% paraformaldehyde solution and DAPI was added to stain the nucleus. Pictures were taken using a fluorescence microscope.
Among the potential prognostic biomarkers for TNBC, increasing attention has been recently center around the role of S100 proteins, a large family of calcium-binding cytosolic proteins tightly linked to inflammatory disorders, neurological pathologies and even progression of diverse tumors, including BC [...