Representative pictures of reproducible effects from two to three independent experiments are shown. B The indicated cell lines were treated with Gefitinib and Paclitaxel at the indicated dose (see Materials and Methods). Cell viability (MTT assay) is presented as the percentage viability of untreated...
Pictures of exosomes derived from MCF-7 and MDA-MB-231 cells were acquired by Tecnai G2 Spirit Bio TWIN transmission electron microscope (FEI, USA). Profiling of circRNA from breast cells and their exosomes TRIzol reagent (Invitrogen, MD, USA) was used for total RNA extracted from breast ...
Then we take pictures and record. Ethics statement Experimental procedures were approved by the Ethics Committee of the Second Affiliated Hospital of Xi’an Jiaotong University and the written informed consent was obtained from all patients before enrolling in the research program. The in vivo assay ...
incubated for 20 minutes at RT and in the dark. Finally, cells were fixed in 1% paraformaldehyde solution and DAPI was added to stain the nucleus. Pictures were taken using a fluorescence microscope.
Pictures were imaged and counted. Cell cycle assays Cells were harvested via trypsinization, washed in ice-cold PBS, fixed in ice-cold 75% ethanol in PBS, centrifuged at 4°C, and suspended in PBS. After, 20 mg/mL propidium iodide (Beyotime, Shanghai, China) was added, and the samples...
H&E staining pictures demonstrated that tumor tissues of HMGPHS NPs plus laser showed higher apoptosis and necrosis (dotted area) cells (Fig. S5A). On the contrary, the other treatment groups retained intact cell morphology, with concentrated chromatin and nuclei. The higher green fluorescence ...
minutes at room temperature for nuclear staining. Lastly, cells were extensively washed with 1X PBS, and YAP nuclear accumulation pictures as well as quantification of nuclear YAP per cell were acquired and analyzed with an Axio Imager Z1 microscope equipped with ApoTome system controlled by ZEN ...
三阴性乳腺癌(TNBC)是指雌激素受体(ER)、孕激素受体(PR)和人类表皮生长因子受体2(HER2)免疫组织化学染色结果均为阴性的乳腺癌亚型,约占所有乳腺癌的15%~20%。TNBC患者通常预后更差,目前主要治疗手段为手术、辅助化疗以及放疗,而很多患者在确诊TNBC时已经错过了手术机会。
会上,诸多研究研究聚焦于探索新兴疗法以提高TNBC的治疗效果。此前已有证据表明,PARP抑制剂可能通过增强肿瘤细胞对DNA损伤的反应而改善肿瘤控制效果。因此,一项临床前研究(P4-03-23)[1]整理和分析了有关T-DXd与PARP抑制剂奥拉帕利联合应用的最新研究成果,旨在揭示潜在作用机制,并为临床实践提供重要指导。本文特此整理...
三阴性乳腺癌(TNBC)占全体乳腺癌的15%～20%,具有侵袭性强,预后差的特点,且以铂类、紫杉烷类、蒽环类及环磷酰胺为基础的新辅助化疗方案疗效与患者长期生存获益仍不尽人意 1,2 。近年来,TNBC患者新辅助免疫联合化疗的探索方兴未艾。在2024 SABCS大会中,一项关于卡瑞利珠单抗联合含铂化疗用于TNBC新辅助治疗的CamReli...