Here, we report a simple and reliable expression and purification strategy based on fusing Tn5 to the small B1 immunoglobulin binding domain of Streptococcal protein G (GB1) and high affinity 10x His tag. The purified recombinant Tn5 showed high DNA tagmentation activity and ultra-low nucleic acid...
Method for efficient soluble expression and purification of recombinant hyperactive Tn5 transposase [ J ] . Protein Expr Purif, 2021, 183: 105866. [23] Paulsen M, Ferguson-Smith AC. DNA methylation in genomic imprinting, development, and disease [ J] . J Pathol, 2001, 195 (1) : 97-110....
Method for efficient soluble expression and purification of recombinant hyperactive Tn5 transposase [ J ] . Protein Expr Purif, 2021, 183: 105866. [23] Paulsen M, Ferguson-Smith AC. DNA methylation in genomic imprinting, development, and disease [ J] . J Pathol, 2001, 195 (1) : 97-110....
One-stop service from gene synthesis and vector construction to protein expression and purification. Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system. Multiple purification systems (30+) to choose. ...
Expression of Tnp mutant proteins for purification was performed in Escherichia coli strain ER2566 (Fλ−fhuA2 [Ion] ompT lacZ::T7 gene1 gal sulA11Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS)endA1 [dcm]) (New England Biolabs). All plasmid propagati...
Herein we report the cloning, expression, purification, and characterization of a soluble form of avian (chicken) Thy-1. A novel isoform of chicken Thy-1 has been cloned which differs from the previously known chicken isoform by eight amino acid residues. Recombinant protein was expressed in ...
After standard alkaline lysis purification (20), the DNA was run on a preparative 1% agarose gel, and supercoiled monomer DNA was cut out of the gel and was prepared with a Geneclean II kit (BIO 101). This protocol avoided the presence of multimeric forms of the plasmid and chromosomal ...
(2) Through several steps, all in the same well, without intermediate purification, genomic DNA is amplified, fragmented, and ligated onto a unique barcode adapter (3) that is merged to all 384 wells, purified, and introduced into Complete Genomics' sequencing platform 10, (4) custom ...