In this study, we designed a novel screening system that combines the TMV (tobacco mosaic virus)-GFP vector and Agrobacterium-mediated transient expression in the model plant Nicotiana benthamiana. This system enables the rapid identification of effectors that interfere with plant immunity....
近日,陕西师范大学生命科学学院植物-微生物互作团队张美祥/安玉艳团队在国际权威学术期刊The Plant Journal在线发表了题为“A robust high-throughput functional screening assay for plant pathogen effectors using the TMV-GFP vector”的研究论文,巧妙借助TMV-GFP载体建立了一种快速、简易、技术门槛低、应用范围广的植...
Using a Tobacco mosaic virus (TMV)-based transient gene expression vector, 30B, which expresses the jellyfish green fluorescent protein (30B-GFP), we describe the predominant types of hybrid viruses that developed in plants. In general, the recombinants deleted the foreign gene and repeated ...
The CaWRKYa, CaMK1, and CaMK2 coding regions were fused to the green fluorescence protein (GFP)-coding region at the N-terminal region in 326 GFP vector. The DNA of GFP-CaWRKYa, GFP-CaMK1, and GFP-CaMK2 constructs was introduced into the hot pepper protoplasts by modified polyethylene ...
Expression of thegreen fluorescent protein-encoding gene from a tobacco mosaic virus-basedvector(Casper andHolt, 1996)绿色荧光蛋白在烟草花叶病毒载体上的表达 摘要 含有GFP的cDNA片段连接在TMV基因组cDNA克隆上,用其体外转录的侵染性RNA转录本侵染烟草叶片。1-2天后,可以在接种叶片和整株植物中观察到绿色荧光...
We selected a well-studied and widely used virus expression vector based on TMV for the delivery of recombinant proteins into the host plant. Co-infiltration of TMV-gfp and binary expression vectors carrying theAt-CycD2andAt-CDC27agenes, respectively, resulted in enhanced GFP fluorescence in agro...
Derivatives of the 30Bcycle3GFP vector that had lost the Cycle3GFP gene were found in several infected cultures and we provide evidence that some vector derivatives were replicating. There were no detectable levels of 30Bcycle3GFP vectors showing loss of insert in directly inoculated cultures ...