关键词:迷迭香酸;高尿酸血症;TMT 蛋白组;尿酸;黄嘌呤氧化酶;腺苷脱氨酶水平;T 细胞分化 中图分类号:R285 文献标志码:A 文章编号:1674 - 5515(2024)06 - 1390 - 08 DOI: 10.7501/j.issn.1674-5515.2024.06.004 Mechanism of rosmarinic acid in treatment of hyperuricemia based on TMT proteomics ...
Therefore, transcriptome sequencing of sperm before and after freezing and thawing might not be the best way to explore the mechanism of sperm freezing damage. Meanwhile, proteins are one of the material bases for sperm function, and have been mainly studied in human medicine at the present ...
(i.e., 126-135Da) in the low mass region of the MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation. Which offers multiplexing capabilities for relative quantitative proteomics analysis. This TMT label technique has been widely adopted in studies ...
This step was followed by the addition of 2 μL of sequencing-grade trypsin (1 μg/μL,Hualishi, Beijing) in each tube, and the solutions were incubated for digestion at 37 °C for 12 h. Finally, the digested peptides were centrifuged at 12000 rpm for 20 min. Then, 50 μL of ...
et al. Transcriptome profiling of bovine milk oligosaccharide metabolism genes using RNA-sequencing. PLoS One 6, e18895 (2011). Article ADS CAS PubMed PubMed Central Google Scholar Download references Acknowledgements The authors acknowledge funding from the National Institutes of Health awards R01AT...
定量蛋白质组学(Quantitative Proteomics)是对一个基因组表达的全部蛋白质或一个复杂混合体系内所有蛋白质进行精确鉴定和定量。可用于筛选和寻找任何因素引起的样本之间的差异表达蛋白,结合生物信息学揭示细胞生理病理功能,同时也可对某些关键蛋白进行定性和定量分析。
sequencing in shotgun proteomic experiments, is not nec- essarily the method of choice for maximal identification of phosphorylation sites. Because of the neutral loss occurring for phosphoserine peptides and, to a lesser extent, for phos- phothreonine peptides, sequencing information is often lo...
ProteomicsSample preparationMass spectrometryPhosphorylationIsotope labelingDespite recent advances in mass spectrometric sequencing speed and improved sensitivity, the in-depth analysis of proteomes still widely relies on off-line peptide separation and fractionation to deal with the enormous molecular complexity...
These patterns cannot be adequately characterized by statistical methods designed for other technologies, such as label-free proteomics or transcriptomics. This manuscript proposes a general statistical approach for relative protein quantification in MS- based experiments with TMT labeling. It is applicable ...
After second acetone precipitation, proteins were digested using rLys-C (Wako) at 37 °C for 3 h followed by sequencing grade trypsin (Promega) digestion at 37 °C overnight at 1 : 50 ratio (enzyme/substrate). Digestion was stopped by the addition of trifluoroacetic acid (TFA), ...