Reagent Preparations Prior to Final Production of concentrate of one step TMB. 1. The resistance of deionized water should be greater than 18 megohm. 2. Preparation of BCD solution. 10 ml deionized water, 2 g, stirred until 2 g BCD is dissolved. ...
substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. The reaction should be monitored and read before the background color becomes too intense ...
biological activityTMB (BM blue) is a color substrate for immunohistochemistry and ELISA. usethis product is a non-carcinogenic substitute for benzidine 1 (negative Ames test), suitable as a peroxidase substrate for enzyme-linked immunosorbent assay. The substrate produces a soluble light blue end ...
The substrate produces a soluble light blue end product that can be read by spectrophotometry at 370 or 620-650nm. The TMB reaction can be terminated with 2MH2SO4 (turning yellow) and the data is read at 450nm. A sensitive and specific reagent used to detect blood, determine hemoglobin, ...