Western Blot (WB), Flow Cytometry (FC/FACS)免疫原 免疫原详情,请参考产品官网页面信息来源宿主 Mouse反应性 人(Predicted Reactivity: 小鼠保存建议 Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire ...
TLR3/Toll-like Receptor 3 Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: ...
Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98016-2-PBS in a different storage buffer formulation.View All Images (3)此产品已下架!产品信息 98016-2-PBS targets TLR3/CD283 in FC (...
The effect of TLR3 agonist, polyinosinic-polycytidylic acid (poly(I:C)), on the growth of human NB cells was evaluated by WST-1 cell proliferation assay, flow cytometry analysis, and immunoblot analysis. Blockade of TLR3 signaling was achieved using TLR3 neutralizing antibody, small interference...
Differentiation potential into adipocytes (middle) or osteoblasts(right) was shown with 400X magnification. Adipocytes or osteoblasts were stained with FABP4 or osteocalcin antibody (green), and nuclei were counterstained with DAPI (blue). 展开 被引量: 3 ...
PE-anti-TLR4/MD-2 antibody was added to BMDMs expressing the TLR4/TLR3 chimera, the cells were incubated for 2 h at 37°C, and then the level of endocytosed TLR4/TLR3 chimera was assessed by flow cytometry. However, no endocytosis of the TLR4/TLR3 chimera was detected by this method...
Adipocytes or osteoblasts were stained with FABP4 or osteocalcin antibody (green), and nuclei were counterstained with DAPI (blue). TLR3 mRNA expression to a plateau level (Fig. 3b). These results suggest that TLR3 expression is more plastic than TLR4 expression following priming of the ...
www.nature.com/scientificreports OPEN Combination of TLR1/2 and TLR3 ligands enhances CD4+ T cell longevity and antibody received: 29 April 2016 accepted: 10 August 2016 Published: 01 September 2016 responses by modulating type I IFN production Bo Ryeong Lee1, Soo Kyung Jeong2, ...
Antibodies and flow cytometry Antibodies and dilutions used in this study are listed in Supplementary Table 2. Antibody staining was performed in the presence of purified CD16/CD32 antibody (Mouse BD Fc-Block™, clone: 2.4G2, #553141, BD Biosciences) at a concentration of 1 µg per...
Flow cytometry analysis revealed that the percentage of M1 macrophages (CD206 negative and CD16/32 positive cells) in the M1-CM-stimulated group was 52.83 ± 3.19%. The M1 percentage was reduced to 5.78 ± 1.27% in miR-26b-5p overexpression group. Conversely, the percentage of...