A mammalian expression plasmid that produced the full-length rTLP1 protein fused with a FLAG tag (DYKDDDDK) at its C-terminus was transfected into COS7 cells. Cells transiently expressing the recombinant proteins were cultured in the presence of [35S]methionine and cysteine. Total cell lysates ...
Putative AsTLPs ranged in size from 204 to 323 amino acids (aa); among them, 13 were hydrophilic and 19-hydrophobic ac- cording to hydrophobicity indicators. All AsTLPs had similar structures, containing an N-terminal signal peptide (21–29 aa), full-length functional catalytic domain GH64-...