The beads were then washed twice with lysis buffer containing 1 m NaCl and twice again with lysis buffer before boiling in 1× SDS sample buffer for SDS-PAGE and Western blotting. SEAP Reporter Assay—-TF-1 cell lines stably expressing the SEAP reporter under the control of NF-κB or SRE...
The activity of HXN-1001 was validated through various in vitro experiments, including blocking TL1A binding to DR3-overexpressing cells, TL1A-induced TF-1 apoptosis, DR3-NFκB-Luc reporter assays, and IFN-γ release from primary T cells. In vivo efficacy was demonstrated in DSS-induced ...
TL1A binding to DR3-overexpressing cells, TL1A-induced TF-1 apoptosis, DR3-NFκB-Luc reporter assays, and IFN-γ release from primary T cells. In... J Huang,H Ran,S Li,... - 《Journal of Crohns & Colitis》 被引量: 0发表: 2025年 Tourmaline as a petrogenetic indicator mineral - ...
METHODS. The binding and blocking efficacy of the bsAb for each target were assessed using various in vitro assays, including competitive ELISA and cell adhesion assays for α4β7, and TF-1 apoptosis and DR3-NFκB-Luc reporter assays for TL1A. The synergistic effect of the bsAb was further...
METHODS. The binding and blocking efficacy of HXN-1003 for each target were assessed using various in vitro assays, including IL23 reporter and mouse splenocyte activation assays for IL23, and TF-1 apoptosis and DR3-NF魏B-Luc reporter assays for TL1A. The synergistic effect of dual ...