Potentiometric setup The potentiometric titrations were performed using an indicator electrode and a reference electrode, in a 10 mm diameter beaker. The buffer solution (1 mM MES pH 7.0) was stirred during the entire experiment with a magnetic stirrer (5 x 2 mm). After obtaining a stable ...
Multiple factors in the preanalytical and analytical steps of a flow cytometry experiment can influence the titration decision and the subsequent ability to resolve populations of interest, requiring careful experimental planning. There are five aspects to consider before starting a titration to ensure the...