Calculation errors Mixing up samples and/or reagents Wrong sample sizes Errors in system setup, instrument operation, or cleaning (not clearing bubbles from the lines, for example) A combination of user training, SOP adherence, and advanced measures for data traceability and integrity will drastically...
Calculation Schema: Titer (TU/ml) = (number of transduced cells in day 1 9 percent of GFP-positive cells 9 1,000/volume of lentivirus used (ul). FACS titer = 30,000 9 0.067 9 1,000/0.07 = 2.8 9 107. Discussion Numerous methods have been described for lentiviral titration, including...
Other than calculation errors, what is one experimental error a student could make while preparing a solution via dilution that would result in the concentration being higher than intended? Your laboratory uses an automated analyzer for quantifying glucose in...