比如BRDU、EDU法;后者主要为MTT、XTT、MTS、CCK-8、WST-1及WST-8法等,在后者中现在最主流的就是CTG(CELL TITER-GLO)发光法,此法可快速灵敏的检测活细胞数量,市场上常用的是Promega公司CellTiter-Glo化学发光细胞活性检测试剂盒。
比如BRDU、EDU法;后者主要为MTT、XTT、MTS、CCK-8、WST-1及WST-8法等,在后者中现在最主流的就是CTG(CELL TITER-GLO)发光法,此法可快速灵敏的检测活细胞数量,市场上常用的是Promega公司CellTiter-Glo化学发光细胞活性检测试剂盒。
The ATP levels were measured with the CellTiter-Glo® kit (Promega, Fitchburg, WI, USA). Hippocampal slice culture We prepared the hippocampal slices (350 μm thickness) from postnatal day 7 Sprague Dawley rats and kept them in culture for 7–13 days in a CO2 incubator at 34°C, as ...
Following 2 min incubation at room temperature, 150μl of lysate was transferred to solid black 96-well plates for luminescence detection using Glomax 96 microplate luminometer (Promega, Fitchburg, WI). The TCID50 was calculated using the Reed-Muench method41. In vitro PBNA. ...
主要包括观察DNA合成含量和检测细胞代谢活性两种,前者主要是DNA前体物质(胸腺嘧啶核苷类似物)掺入法,比如BRDU、EDU法;后者主要为MTT、XTT、MTS、CCK-8、WST-1及WST-8法等,在后者中现在最主流的就是CTG(CELL TITER-GLO)发光法,此法可快速灵敏的检测活细胞数量,市场上常用的是Promega公司CellTiter-Glo化学发光细胞...