Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing grade modified trypsin (Sigma) after four-fold dilution in water. Protease digestion was stopped by addition of trifluoroacetic acid and precipitates were removed by centrifugation. Peptides were purified using reversed-...
ChRCC, chromophobe renal cell carcinoma; LGG, low-grade glioma; PRCC, papillary renal cell carcinoma; TCGA, The Cancer Genome Atlas. Download: Download Acrobat PDF file (74KB) Supplementary Figure S10. Gene-set enrichment analysis results of clear cell renal cell carcinoma Gene-Set Enrichment ...
To 9.0 liters of the 50mM citrate buffer solution prepared in Step 1, add 6.0 milliliters of 50% Biological Grade Glutaraldehyde Bring the solution volume up to 10.0 liters using the 50mM citrate buffer solution prepared in Step 1. Step 3) Adjust the pH of the solution to 6.40 + 0.05...
The qRT-PCR mixture contained 125 ng of cDNA from each sample, primers to a final concentration of 50 μM each, 12.5 μl of the SYBR Green PCR Master Mix (Applied Biosystems, USA) and PCR-grade water up to a total volume of 25 μl. Each gene reaction was performed in technical ...
Low-grade serous and CCC subtypes are known to be relatively chemoresistant [3,4,6,7]. Accordingly, greater understanding of the relationship between the heterogeneity of EOC and the underlying biology should result in promising new treatment strategies. Tissue factor (TF) is...
This capacity for single cell immortality was common amongst ancestral eukaryotic organisms, like protists, but is comparably scarce within tissue-grade organisms. Additionally, aggregations of the immortal replicative cancer cells demonstrate the same dynamic cell fate turnover that provide basal metazoans...
(100 mg/mL) Qiagen p/n 19101 DNeasy Blood & Tissue Kit Qiagen p/n 69504 Proteinase K (>600 mAU/mL, solution) Sodium thiocyanate (NaSCN)† Qiagen p/n 19131 Sigma-Aldrich 467871-50G Ethanol (95% to 100% molecular biology grade) Sigma-Aldrich p/n E7023-6×500ML Tween 20† Sigma...
(1,4′-isopropylidene diphenylene carbonate), vinylidene chloride, acrylonitrile copolymer, vinyl chloride-diethyl fumerale copolymer, butadiene/styrene copolymers, silicone rubbers, especially the medical grade polydimethylsiloxanes, ethylene-propylene rubber, silicone-carbonate copolymers, vinylidene chloride-...
250 mM D-Sucrose (Fisher Scientific, cat. no. BP220-1) 10 mM Tris-HCl, pH 7.5 (Mediatech Inc., cat. no. 46-030-CM) 1 mM MgCl2 (Ambion, cat. no. AM9530G) Molecular Biology Grade sterile H2O to 500 ml • Phosphate buffered saline (PBS; ThermoFisher, cat. no. ...
ALBI grade Journal of Hepatology, Volume 70, Issue 4, 2019, p. 808 Teh-Ia Huo,…, Chia-Yang Hsu Decision making in systemic therapy of hepatocellular carcinoma: Should we pay attention to disease aetiology? Journal of Hepatology, Volume 75, Issue 4, 2021, pp. 763-764 Peter R. Galle, ...