Aging is associated with multiple cellular changes that are often tissue specific1. Cytosine methylation, however, stands out, as it allows for the development of pan-tissue aging clocks (multivariate age estim
Fig. 1: Characteristics of samples in the pilot phase of PigGTEx project. a, Clustering of 7,095 RNA-seq samples based on the normalized expression (log10-transformed TPM) of 6,500 highly variable genes, defined as the top 20% of genes with the largest s.d. of TPM across samples. b...
In particular, somatic cell turnover is pervasive in mammalian tissues, but the dynamics of birth, death and replacement are difficult to measure. For example, in the intestine, small numbers of mitotic epithelial stem cells maintain intestinal crypts and undergo random turnover such that only one...
Effect of methotrexate on homocysteine and other sulfur compounds in tissues of rats fed a normal or a defined, choline- deficient diet. Cancer Chemother Pharmacol 1988;21 :313-318.Svardal AM, Ueland PM, Berge RK, et al. Effect of methotrexate on homocysteine and other sulfur compounds in ...
Orthogonally, these same tissues were characterized by multiround immunofluorescence that defined expression of CA19-9 and sTRA as well as other lectins toward carbohydrate epitopes with the potential to improve PDAC diagnosis. These analyses revealed distinct differences not only in N-glycan spatial ...
Furthermore, as shown in the “Results” section below, only a comprehensive analysis allows comparison across these classes. Complete details of ROP, including all parameters and thresholds used, are provided in the Additional file 1: Supplementary Methods....
However, current tissue engineering strategies struggle in the attempt to create patterned constructs with biologically distinct functionality. In this work, a developmentally-inspired modular approach is proposed, whereby distinct cartilaginous organoids are used as living building blocks. First, a ...
Also the molecular pathways enriched in STCs or LTCs are clearly different (Table 5): genes overexpressed in STCs vs LTCs are enriched in KEGG pathways such as “cell cycle”, and, significantly, “TGF-beta signaling pathway”. On the contrary, and in agreement with the cellular component...
With these and other objects in view, the inveni tion comprises the various features hereinafter described and explained and more particularly defined in the claims. While the coating composition of my invention has particular utilization when applied to organic tissues such as skin tissue as a pro...
We applied a false-discovery rate (FDR) threshold of 0.01 and an age coefficient threshold of 0.005 (corresponding to an approximately 10% fold change). Comparison between bulk and single-cell datasets The differential gene analysis was defined on a per tissue basis. First, we investigated genes...