The fluorescent antibody tissue stain for treponemes is described and its usefulness in experimental ocular syphilis documented. Two primates, inoculated by intraocular and intracisternal routes, respectively, acquired syphilis serologically but only one developed clinical lesions. The fluorescent antibody tissu...
In normal colon tissue, it is composed of vascular, lymphatics, submucosal plexus and mostly two distinct collagen fiber populations in a cross-ply arrangement (Fig. 1c). The colon muscularis consists of two smooth muscle layers; outer longitudinal and inner circular separated by the myenteric ...
Wells JA, Smith JL. The fluorescent antibody tissue stain for Treponema pallidum in the eye. Am J Ophthalmol. 1967 Mar; 63 (3):410–418.Wells, J. A., and Smith, J. L. : The fluorescent antibody tissue stain for Treponema pallidum in the eye. Am. J. Ophth. 63:410, 1967....
Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin,
while preserving limb function as best possible. The minimal free margin to be considered adequate may depend on many factors, including histological subtype, pre/postoperative therapies, and the nature of resistant anatomical barriers, i.e., muscular fascia, vascular adventitia, periosteum, and epine...
Millicell® 24 well hanging cell culture inserts pore size 3.0 μm, clear PET membrane, diam. 6.5 mm, Tissue Culture (TC)-treated surface, sterile; Synonyms: permeable inserts, tissue culture inserts, boyden chamber, transparent membrane insert at Sigm
Berner PF, Frank E, Holtzer H, Somlyo AP (1981) The intermediate filament proteins of rabbit vascular smooth muscle: immunofluorescent studies of desmin and vimentin. J Muscle Res Cell Motil 2:439–452 Article CAS Google Scholar Böck P, Breitenecker G, Lunglmayr G (1972) Contractile fi...
Secondary fluorescent anti-mouse antibody was from Molecular Probes (used at1:1,000, Invitrogen). Chick blood vessels were visualized by using fluorescein-coupled Sambucus nigra lectin (SNA-1 lectin, 1:100, Vector Laboratories). Cell nuclei were visualized by DAPI (Invitrogen). Microphotographs ...
All fluorescent images were acquired using a TCS SP8 confocal laser-scanning microscope and processed with Las X software. Full size image Discussion In this study, we propose a one-step reagent, MAX, to process a wide variety of biological specimens using a multistep combination. MAX is a ...
Sections used for immunofluorescent staining were incubated with primary perilipin (Abcam, UK), CD31 (Abcam, UK) antibody and subsequently respectively incubated with FITC- and PE- conjugated goat anti-rabbit IgG antibody (Servicebio, China). Nuclei were counterstained with DAPI stain. Moreover, a...