In these bioartificial structures, the tissue compartments must be scaled to mimic nutrient transport existent within natural capillary beds found in the body. Generally, these in vivo beds are composed of 100 μm thick tissue slabs sandwiched by vasculature. Thus, the microenvironment of ex vivo ...
. We found that inks comprising a total polymer concentration below 15 w/w % are prone to spreading, which compromises shape fidelity, whereas inks with polymer concentration exceeding 25 w/w % are difficult to extrude due to clogging in the printing nozzles. Inks within the intermediate range...
Here, we provide the first direct evidence in the intact heart that the cells in myocardial scar tissue are electri- cally coupled to the surrounding myocardium and can support the passive conduction of action potentials. Using models of cardiac injury that produced transmural scars, we demonstrate...
In developing human heart tissues, perlecan first was found in the heart anlage, then in the basement membranes of the endo- and pericardium, as well as capillaries, through the first twelve weeks of growth (Roediger et al., 2009). 4.2. Bone and cartilage In mineralized bone itself, there...
Notably, Peng and colleagues found that co-culturing bone marrow mesenchymal stem cells (BMSCs) with ginseng-derived nanovesicles resulted in the internalization of numerous miRNAs from the PDNVs into the BMSCs. These specific miRNAs were found to target genes associated with the regulation of ...
In the present study, we found that the ferroptosis of adipose tissue cells caused tissue wasting in experimen- tal models of lung cancer cachexia. Mechanistically, we demonstrated that wasting tissue had an increased number of tissue-infiltrating neutrophils (TI-Neu), and showed that these cells ...
We found that the development and function of gastric or intestinal organoids grown in tissue extracellular matrix hydrogels are comparable or often superior to those in Matrigel. In addition, gastrointestinal extracellular matrix hydrogels enabled long-term subculture and transplantation of organoids by ...
4A) [121]. Similarly, micro-channeled hydrogel patch was bio-printed to treat infracted patients [122]. Further, multicellular spheroids were printed onto a needle array to make a tubular construct to develop a heart tissue [123,124]. This tissue was used to assess the therapeutic response,...
Of these, Caveolin-1 is enriched in the plasma membrane and is commonly found in lipid rafts (Jasmin, Frank, & Lisanti, 2012). Because Caveolin-1 is abundant on the cell surface, it provides an excellent target for staining and imaging the plasma membrane of adipocytes. Lipid droplets are...
The PEGASOS passive immersion procedure could clear soft tissue organs in a much shorter time because decalcification is not needed. Within 7 days, we were able to make the brain, liver lobe, spleen, heart, kidney, stomach, intestine, and lung completely transparent (Fig. 2f; Supplementary Info...