The present disclosure is a tissue-embedded section preparation method in which a control unit controls an operation of preparing an embedded section of an embedded block in which a tissue is embedded and an operation of collecting the embedded section by a chip by a microtome. The control unit...
The feasibility of extrusion-based 3D co-printing is verified through mold-assisted structured ink preparation and process parameter optimization. To determine the design parameters including structured ink height and cross-section, both structured inks with material distribution of core–shell and symmetric...
method and tissue-embedded section preparation device 发明人：万里 千裕,坂本 健,小木 修,野田 英之,齋藤 博樹 申请号：JP2019110163 申请日：20190613 公开号：JP2020201206A 公开日：20201217 专利内容由知识产权出版社提供 专利附图：摘要：PROBLEM TO BE SOLVED: To propose a technique capable of recovering...
Adamstone, F. B. and Taylor, A. B. 1948 . The rapide preparation of frozen tissue section. Stain technol. , 23: 109 [Taylor & Francis Online]ADAMSTONEF,. B., and TAYLORA, . B. The Rapid Prep aration of Frozen Tissue Sections. Stain Technol., 23: 109-16, 1948....
Tissue section sample is sealed storage at 4°C | V Xylene (1) 10min | V Xylene (2) 10min | V Xylene (3) 10min | V 1/2 Ethanol 1/2 Xylene 15min | V 100%Ethanol 5min | V 1/2 Xylene 1/2 Paraffin 30min | V 95% Ethanol 5 min ...
Tissue preparation 1. Dewax/deparaffinize and rehydrate the FFPE tissue according to standard IHC-P (paraffin) protocols. 2. Perform heat-induced epitope retrieval (HIER) using either Citrate Buffer (pH 6.0) or EDTA (pH 9) using a microwave or pressure cooker according...
Tissue section preparation, EdU staining, and immunohistochemistry The experimental mice were anesthetized by intraperitoneal injection of 4% chloral hydrate and then transcardially perfused with saline followed by 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS). Mouse brains were dissected, ...
Preparation of the 3D printable tissue adhesive ink The dried PU-PAA was redissolved at a concentration of 20 w/w % in 70% ethanol and mixed in a 25:2 (v/v) ratio with a solution comprising 33.3 w/v % EDC and 33.3 w/v % NHS in 70% ethanol to yield around 10% (mol/...
However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells. ...
and Sharon Wheeler and medical laboratory assistant Liz Marra for sharing their knowledge and experience; Joelle Maclean for secretarial support; Juanita Meyer for assistance with literature search; Xavier Wehrli for illustrations; and LHSC histology section head Dr. Keith Kwan for many valuable insights...