One or more nucleic acids are then amplified using PCR and detected across the tissue to produce a map. As an initial proof of concept, a DNA map was made from a frozen tissue section using 2D-PCR at the resolution of 1.6 mm per well. The technique was improved to perform the more ...
Section 2: dataset pre-processing Channel crosstalk removal Similar to fluorescence imaging, mass-spectrometry imaging such as MIBI also has channel crosstalk due to the formation of adducts6 or isotopic impurity of the elemental labels used. Thus, Rosetta algorithm was applied to extracted raw images...
The Bregma distance is given for each imaged brain section. Cells are colored based on the cell-type annotation from the original study17. b, Left, the 9, 10, 12, 12 and 11 hypothalamic nuclei or regions in the images were manually outlined by the authors of the original study. Right,...
Hormones and growth factors may be incorporated into the scaffold itself and released over time, or the cells in the construct can be modified genetically (see section on genetic engineering) or otherwise to produce the growth factors themselves. In addition, the recipient's own tissue surrounding...
Formally, an n-dimensional deterministic dynamical system is traversed by a surface of section S with dimension (n−1) that is traverse to the flow, e.g., all trajectories starting from S flow through it and are not parallel to it. Then a Poincare map is a mapping from S to itself ...
Regulated under section 361 of Public Health Service Act Higher risk “351” Allogeneic unrelated donors and/or more than minimally manipulated and/or non-homologous use Regulated under section 351 of Public Health Service Act, and subject to same rules as drugs & other biologics for IND and pre...
Shown is a 3D reconstruction of developing cranial blood (contrasted by IB4-Alexa Fluor™ 647, green) and lymphatic vessels (endogenous fluorescence, red) of an optically sectioned embryo at E14.5 after CUBIC-based tissue clearing. Download: Download video (4MB) Video S2. Light sheet ...
Brains were cryoprotected by placing into 30% sucrose for 3–4 days before being sectioned using a cryostat (Leica) at 50 μm thickness. Sections were then washed three times with PBS before being incubated in blocking solution (PBS + 0.5% Triton X100, Sigma +10% Normal Goat Serum...
1A, B. First, chemically fixed tissue section, which can be immunostained if needed, is treated with N-succinimidyl acrylate to install acryloyl group on proteins primary amine groups thus enabling their reversible anchoring into polymer mesh via amide group. The treated sample is then embedded ...
Tumors are cryosectioned and stained for the degree of hypoxia (pimonidazole), proliferation activity (KI67), apoptosis (DNA strand breakage, TUNEL), CD31 for microvessel density, perfused microvessel density (co-localization of Hoechst 33342 and CD31), and necrosis (based on histologic assessment ...