The collagens of one cell layer are cross-linked to those of the adjacent cell layer to provide stable coupling between the layers of cells. In addition, proteoglycans are bound to the basal adhesion molecules, to the collagens, and/or to the basal cell surface. The lateral and basal ECM...
New homeostasis re-establishment after wound was assessed on the basis of the following features: (1) complete re-epithelialization, assessed through an haematoxylin and eosin (H&E) staining of skin sections; (2) re-establishment of comparable epidermal differentiated cell layers with 0w, where the...
Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to-batch variation, high cost, and safe...
2016). We noticed that the density corresponding to the collected EV layers in different studies varied greatly, from 1.06 to 1.19 g/ml, as shown in Table 2. In addition to possible differences in individual laboratory testing methods for density, different tissue sources can also affect the ...
The emergence of large visual databases such as ImageNet, including more than 10 million images and more than 20,000 classes [17], enables CNNs to provide rich and diverse feature description from general images. Responses of CNN hidden layers provide different levels of image abstraction and ...
Depending upon the processing technique used, the two constituents can be either randomly inter-connected, bi-continuous phases, or the constituents could have a gradient-like architecture in the form of a laminate-type composite with a well integrated interface between the two constituent layers. ...
Normal wound healing is a dynamic and complex multiple phase process involving coordinated interactions between growth factors, cytokines, chemokines, and various cells. Any failure in these phases may lead wounds to become chronic and have abnormal scar
According to the American Society of Breast Surgeons, standard guidelines for BCS involve “closing the surgical defect in layers as cosmetically as possible” following resection of the tumor6. Healing of the complex surgical wound follows, initially with a seroma or hematoma forming in the defect...
State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire mammals. When combined with light-sheet microscopy and automated approaches to image analysis, existing tissue-clearing methods can speed up and may reduce...
This patient-specific graft requires a 6-9 month culture period in which the autologous fibroblasts produce sheets of tissue. The sheets are fused together around a stainless steel mandrel (4.8 mm diameter), inner fused layers are dehydrated, and the graft lumen is seeded with autologous ECs (...