NOTES ON SOME EXPERIMENTS WITH SNAIL TISSUE IN CULTUREdoi:10.1038/icb.1935.7BourneGeoffrey
Notes on disease resistance of tissue culture sub-clones and fusion of sugarcane protoplasts 来自 AGRIS 喜欢 0 阅读量: 23 作者: M Krishnamurthi 被引量: 37 年份: 1975 收藏 引用 批量引用 报错 分享 全部来源 求助全文 AGRIS 相似文献 引证文献...
Ronawk’s Bio-Blocks™ enable cell culture in a 3D, tissue-mimetic environment, which allows cells to form intricate 3D structures and tissue-like aggregates. The more true-to-life microenvironment of the Bio-Blocks drives cells…
Despite subsequent research efforts by other groups, such as attempts to induce differentiation using a three-dimensional culture system [85], there remained limited progress in the functional maturation of the induced SGN-like cells. Glutamatergic primary auditory neurons possess a large number of ion...
Differentiated adipocytes cultured in 6 well dishes were trypsinised, washed and suspended in 1 ml of cell culture medium containing 100 nM Mitotracker Green (Molecular Probes) and incubated for 30 min at 37 °C protected from light. Stained cells were washed and analysed for green fl...
Previous work also showed that EVs isolated from cell culture had significantly different NAD(P)H fluorescence lifetime than parent cells, but with a lifetime profile most similar to that of the parent cell cytosol, and less similar to the parent cell nucleus and mitochondria25, indicating that...
After 48 h, the supernatants and cells in the culture were harvested for western blot and the enzymatic activity assay. MC3T3-E1 cell lines were obtained from the American Type Culture Collection (ATCC, CRL-2593) and maintained in alpha-modified Eagle’s medium (α-MEM; Hyclone, USA) ...
The shells were then cracked and the contents transferred to 10 cm cell-culture Petri dishes. Embryo culture was continued for a another 7 days, when two injuries to the CAM were inflicted by parallel scalpel superficial cuts of 1 cm area, with a subsequent scarping off the epithelium of ...
Culture of human corneal epithelial cells (HCE-2 50.B1 cell line, lot no. 70015331, ATCC) was established according to the manufacturer’s instructions. In brief, serum-free keratinocyte growth medium (1×, Gibco) was supplementedl-Glutamine, 5 ng ml−1epidermal growth factor and 0.05...
Co-expression of basic helix–loop–helix protein (bHLH) and transcriptional activator-Myb genes induced anthocyanin biosynthesis in hairy root culture of Nicotiana tabacum L and Ipomea tricolor. Acta Physiol. Planta 39, 59 (2017). Article Google Scholar Gonzalez, A., Zhao, M., Leavitt, J. ...