96 wells in total. Each well will hold slightly more than 300 μl. Alternatively, if large numbers of cells are required, for example to propagate a stock culture of virus, then a Winchester bottle can be used.
For evaluation of in vitro cytotoxicity, NIH3T3 cells were seeded in 96-well tissue culture plates at a density of 1 × 104cells per well. After incubation for 24 h, the cells were washed with PBS. Then, 0.2 ml of the polymer solution was added to each well and the plates...
We observed that PSCs had low cell proliferation rate in the 3D culture environment. To deal with this problem, a p53 (a well-known tumor repressor) inhibitor (Pifithrin-α) and a Yap (a positive regulator in cell survival and proliferation) activator (XMU-MP-1) were applied in the cultu...
峰源隆国际贸易(上海)有限公司主要致力于“96孔细胞培养插入板 Corning HTS Transwell -96 Tissue Culture Systems ”的生产销售。多年的“96孔细胞培养插入板 Corning HTS Transwell -96 Tissue Culture Systems ”生产与销售的经验,与各行业新老用户建立了稳定的
Fig. 2: Visual representation of a TCID50 assay on a 96-well microplate Advantages of TCID50 assays performed on microplate readers The performance of virus quantification and infectivity experiments, such as 50% Tissue Culture Infectious Dose (TCID50) assays, on a microplate reader of...
A DNA assay was performed by loading samples in duplicate into a 96-well plate along with calf thymus DNA standards (Sigma). The samples were incubated for 15 minutes at room temperature with 0.1 μg/mL Hoechst 33258 dye (Sigma) suspended in 10 mM Tris-HCl buffer with 1 mM EDTA and ...
4000 cells were seeded in each of the 96 well-plate, were previously placed the different composite membranes and cultured with α-MEM + 10% SFB for 44 h before. After that, 20 µL of culture medium was replaced with 20 µL of CellTiter 96 Aqueous One Solution Cell ...
Most 3D models are amenable to high content imaging as well as biochemical endpoints. 3D Microtissue, Spheroid & Multi-layer Cell Model Services High Content Imaging of 3D Microtissues ATP Content in 3D Microtissues 3D Hepatotoxicity 3D Hypertrophy Assay ...
RT-PCR Assay (48) LE ZQIAGEN 1061108 AIV-H9 RT-PCR REAGENTQIAGEN 9016623 BR MDx CTM 96 PCR Setup, PROQIAGEN 9016587 Microplte Washer, RCS1QIAGEN 9016515 Bucket Set (2), for microplate, sealableQIAGEN 9019856 36-well High Profile RotorQIAGEN 9019660 Torx key set, PyroQ96VWQIAGEN 9019646 ...
For Myogel assay, the 96-well Transwell plate was coated with 50 μl Myogel (0.5 mg/ml) and left to settle overnight in the cell culture incubator. The excess of the coating was removed carefully with suction, and 1000 cells in 100 μl media per well were added. The cells ...