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Procedurally, sterile fluid, preferably isotonic saline solution, is injected into the tail just distal to where the hemipenes is located if the individual is a male. The fluid, as much as 100 mL in large specimens, is injected into the tail until either sufficient eversion has occurred or ...
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Samples of 10 adult male and five female nematodes were washed three times with pre-chilled PBS buffer and incubated for 30 min with 1 mm EZ-link Sulfo-NHS-SS-Biotin (Thermo scientific), or PBS only (negative control), at 4 °C with gentle agitation. The biotinylating solution was remove...
We should work hard to be safe women for each other. When there are interpersonal problems and questions, we should be careful to talk only with the people who are part of the problem or the solution. I wish I would have said that because it’s what my parents taught me and it works...
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The control group rats received only tween 20 solution (1%, v/v) as vehicle. Prior to commencement of the experiment, all rats were weighed, marked for identification, and fasted overnight but were allowed free access to water. After dosing, the rats have further fasted for 4 h and ...
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Together with its Enea Openwave Traffic Management components, Enea provides a best-in-class solution to classify, optimize and steer network data traffic utilization, combining the policy manager on control plane and the enforcement in the user plane. ...