Then, we tested if the R14G PTEN sensor could be used for time-lapse imaging in HEK cells and permit dynamic measurements of PTEN activity in the same cells over time. Single HEK cells displayed a robust decrease in lifetime following EGF application (Fig.2d–f). The same cells then disp...
Measurements involving specific cell type isolation or monitoring lineage dynamics using time-lapse microscopy will allow a more precise evaluation of cell proliferation rates. Finally, the precision of the estimated breakdown of embryonic cells into types per time point is based on thresholding a ...
Going beyond a single pair of time points, we combine moslin’s couplings across all time points to studyC. elegansembryogenesis using CellRank 2 [23], a computational fate mapping tool. To account for developmental asynchrony within each time point, CellRank 2 computes, for each time point, ...
K. Deep learning as a predictive tool for fetal heart pregnancy following time-lapse incubation and blastocyst transfer. Hum. Reprod. 34, 1011–1018 (2019). 23. Bodri, D. et al. Influence of different oocyte insemination techniques on early and late morphokinetic parameters: retrospective ...
& Wang, X. Celltracker: an automated toolbox for single-cell segmentation and tracking of time-lapse microscopy images. Bioinformatics 37, 285–287 (2021). Tsai, H.-F., Gajda, J., Sloan, T. F., Rares, A. & Shen, A. Q. Usiigaci: Instance-aware cell tracking in stain-free ...
Increasing the period P implies larger lapse of time in which the mechanotransduction is switched on. Conse- quently, a larger number of events are expected. If the dynamics of the system does not change during the oscillations of the perturbation, then the area under the histograms increases ...
lapse morphokinetic embryo selection and pre-implantation genetic testing for aneuploidy (PGT-A). However, the treatment methods for endometrial factor infertility remain rudimentary16. Although bulk transcriptomic studies have identified displaced WOI and in-phase pathological gene signatures of RIF17, ...
Execution time, aka wall clock, refers to the time a computer takes to execute statements from the HDL code during simulation. It is of little interest to circuit designers as long as their simulation runs complete within an acceptable lapse of time. ...
Two-photon time-lapse imaging of fusion and fission of vesicles containing BChol. CHO cells were pulse-labeled with BChol followed by a chase to obtain the steady-state distribution. The cells were placed on a temperature-controlled stage of a home-built 2P-microscope maintained at 35 ±...
1. A system for generating time-lapse videos, the system comprising: an image sensor configured to generate a visual output signal conveying visual information based on light that becomes incident thereon, the visual information defining visual content; an optical element configured to guide light wit...