9:00 am in Llangefni, United Kingdom is 3:00 am in Coralville, IA, USA Llangefni to Coralville call timeBest time for a conference call or a meeting is between 2pm-6pm in Llangefni which corresponds to 8am-12pm in Coralville 9:00 am GMT (Greenwich Mean Time) (Llangefni, United King...
Coralville Walford Belle Plaine University Heights North Liberty New Sharon Iowa City Fairfax Maharishi Vedic City Oskaloosa Lone Tree Atkins Fairfield Ely Solon Grinnell Eddyville Ottumwa West Branch Columbus Junction Tama Cedar Rapids Palo Hiawatha Toledo West Liberty...
DNA for double eGFP with a (GGGGS)3 linker, where G refers to glycine and S to serine, was synthesized as a double-stranded DNA gBlock (Integrated DNA Technologies, Coralville, IA), and cloned into pET151 according to manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA). ...
DNA template (5′-GACGTACGTGATCGCA-3′, T) and primer (5′-GCGATCACGT-3′, P) were purchased from IDT (Coralville, IA) and were used without further purification. The P/T duplex was prepared by combining equal amounts of the primer and template strands in Bst buffer supplemented with...
These gene types have been used widely as reference genes in studies of insects and fungi. Primers were designed based on the sequences selected by the PrimerQuest software (Integrated DNA Technologies, Inc, Coralville, IA, USA) (Table 1). Additionally, BLAST analyses were performed to verify...
这些合成寡核苷酸购自Integrated DNA Technologies(Coralville,IA,USA),其核苷酸序列可在补充部分中获得。分析每个寡核苷酸集以预测杂交能量和潜在的二聚体形成,并使用DI核酸杂交和熔融预测进行说明(DINAMelt Server,RNA Institute,SUNY Albany,Albany,NY;可在http://mfold.rna.albany.edu)[18]。用于实时NASBA的试剂及其...
For the design of Lux™ and Plexor™ assays, online primer design software D-Lux™ Designer (Invitrogen) and Plexor™ Primer Design Software (Promega) were used, and the primers were synthesized by Invitrogen and IDT (Integrated DNA Technologies, Coralville, IA), respectively. The ...
The primers were synthesized by Integrated DNA Technologies (Coralville, IA), and the probe was synthesized by BioSearch Technologies (Novato, CA). Primers and probe were utilized in a 25 μL reaction containing 12.5 μL of Quantitect Probe RT-PCR 2× mix (Qiagen, Valencia, CA), 0.25 μ...
Current local time in New London, IA, USA. Time zones CDT, Central Daylight Time, America/Chicago. New London UTC/GMT offset, daylight saving, facts and alternative names
Coralville Solon University Heights Iowa City Ely West Branch Fairfax Walford Mount Vernon Lisbon Cedar Rapids Riverside Kalona Marion Lone Tree Hiawatha Mechanicsville West Liberty Atkins Williamsburg Robins Springville Wellman Palo Tipton Marengo Anamosa North English...