We found cytokine production, cell junctions, the nuclear transcription factor-κB (NF-κB) signalling pathway, and inflammatory responses associated with the differentially expressed genes, as revealed by Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analyses. Se ...
KEGG analysis reveals that JEV infection modulates tight junction signaling by hypermethylation of theAfdnpromoter. (A) KEGG pathway analysis of DMGs in upstream2k region. The ordinate represents the enriched pathways, and the abscissa represents the Rich factor of the correspond- ding pathways; the ...
The claudin family of proteins is integral to the tight junction (TJ), the apical cell-cell adhesion and a key regulator of the epithelial barrier. Using comprehensive physiological and biochemical analysis of intestinal and renal tissues from high-fat diet fed mice, critical for maintaining ...
Cell proliferation, apoptosis, differential gene expression and KEGG pathway analysis, intestinal paracellular permeability, intestinal morphology, and expression of HSP and TJ proteins, and p-AMPK were determined. l-Arginine promoted cell proliferation and reduced apoptosis after heat exposure at an ...
EnrichR pathway analysis resulted in 13 significantly-enriched KEGG pathways (p < 0.01) of which seven showed a large number of overlapping genes. Several genes were involved in intracellular trafficking, possibly playing a role in nutrient transport, POS phagocytosis or membrane protein trafficking, ...
Cell proliferation, apoptosis, differential gene expression and KEGG pathway analysis, intestinal paracellular permeability, intestinal morphology, and expression of HSP and TJ proteins, and p-AMPK were determined. l-Arginine promoted cell proliferation and reduced apoptosis after heat exposure at an ...
plantarum MB452, 19 were involved in tight junction formation (Table 1). Analysis of KEGG pathways using EASE showed that the tight junction pathway was one of four pathways that was enriched with differentially expressed genes (P and global FDR < 0.05; Additional File 1 Table S3). The ...
et al. Potential probiotic Lactiplantibacillus plantarum strains alleviate TNF-α by regulating ADAM17 protein and ameliorate gut integrity through tight junction protein expression in in vitro model. Cell Commun Signal 22, 520 (2024). https://doi.org/10.1186/s12964-024-01900-7 Download citation...
SPA was conducted by nanoLC-MS/MS, while the identified proteins were mapped on to Gene Ontology and KEGG pathway components analysis. The protection of HSYA for blood-brain barrier in mice with ischemia stroke was assessed with the leakage of Evans Blue. The expression of tight junction ...
The TEER assay is a quantitative technique to measure the integrity of tight junction dynamics with in vitro models. It was performed as described previously [30]. Briefly, caco-2 cells were grown on polyester transwell inserts (Corning, Germany) with a pore size of 0.4 µm2. The confluent...