Partial purification was performed on CMCV and FCV. Five T175 flasks per virus were seeded with BSR cells at 80% confluence 1 day prior to infection. Cells were infected with CMCV or FCV at a MOI of 0.1 for 1 h at room temperature with gentle agitation. Inoculum was removed and replace...
Method for quick test diagnostics deals with applying 15-20 mcl tested material onto a slide, adding equal amount of a certain preparation necessary for express diagnostics followed by thorough mixing by shaking a slide to visually determine the presence of antigen to tick-borne encephalitis virus ...