During the 2-hour in vitro transcription, Cy3- or Cy5-labeled CTP was incorporated into each amplified RNA (cRNA). Products of the reaction were then purified using RNAeasy mini spin columns (Qiagen, Dusseldorf, Germany). Hybridization and slide and image processing were carried out according ...
14–17 Because of the lack of agreement on the minimal nuclear alteration criteria required to diagnose papillary carcinoma, many nodules previously diagnosed as follicular adenoma have in the past years been labeled as encapsulated (non-invasive) follicular variant papillary carcinoma. Since the year ...
The Metabolism of S35-Labeled Antithyroidal Substances in the Thyroid Gland and Other Organs of Cockerels, with Particular Reference to the Nucleic Acid Fractionsdoi:10.1530/acta.0.0180229Many investigators have given considerable thought to the mechanism of antithyroidal substances. For a review cf. ...
receptors. Animal studies show that thyroid hormones play a biological role mainly by binding to TR and TR-α in knockout mice. Liver insulin sensitivity is improved in the body, and hepatic gluconeogenesis is inhibited. Feng et al used T3 to treat hypothyroidism mice labeled with fluorescent ...
3 for 1 week prior to the oral dose of 14C-labeled material. The results of these studies and others suggest that a primary (direct) mechanism of action of FD&C Red No. 3 on the thyroid is unlikely because of (1) the failure of the dye to accumulate in the gland, (2) consistently...
T cells were labeled with 5 μM carboxyfluorescein succinimidyl ester (CFSE) in complete RPMI and the proliferation was evaluated by flow cytometry 72 h later. RNA-seq analysis and Real-Time quantitative PCR (RT-qPCR) Total RNA of cancer cells or tumor tissues was isolated using TRIzol ...
F Schematic diagram of the zebrafish models. G The tumorigenesis of zebrafish in control and ISG15-KD groups. H The metastasis of zebrafish in control and ISG15-KD groups. Red fluorescence represents labeled ATC tumor cells Full size image Simultaneously, we also confirmed the inhibitory effect ...
Following this, the cells were incubated with Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H + L) (A0423, Beyotime Biotechnology, China) for an additional hour at room temperature. Subsequently, the nuclei were subjected to staining with DAPI for a duration of 10 min, followed by ...
Pharmacological protection of the thyroid gland against radiation damage from radioactive iodine labeled compounds in children: a systematic reviewThyroid damageThyroid cancerHypothyroidismI-131I-123Radioactive iodineClinical and Translational Imaging - There is currently no consensus on which protective ...