we cut the labeled area into non-overlapping tiles of 224*224 pixels in size. Tiles were filtered with the criterion of 50% or more of class-labeled components to obtain a dataset with a high degree of lesion fit from both WSI and tile-level perspectives. We use our deep learning...
During the 2-hour in vitro transcription, Cy3- or Cy5-labeled CTP was incorporated into each amplified RNA (cRNA). Products of the reaction were then purified using RNAeasy mini spin columns (Qiagen, Dusseldorf, Germany). Hybridization and slide and image processing were carried out according ...
14–17 Because of the lack of agreement on the minimal nuclear alteration criteria required to diagnose papillary carcinoma, many nodules previously diagnosed as follicular adenoma have in the past years been labeled as encapsulated (non-invasive) follicular variant papillary carcinoma. Since the year ...
F Schematic diagram of the zebrafish models. G The tumorigenesis of zebrafish in control and ISG15-KD groups. H The metastasis of zebrafish in control and ISG15-KD groups. Red fluorescence represents labeled ATC tumor cells Full size image Simultaneously, we also confirmed the inhibitory effect ...
T cells were labeled with 5 μM carboxyfluorescein succinimidyl ester (CFSE) in complete RPMI and the proliferation was evaluated by flow cytometry 72 h later. RNA-seq analysis and Real-Time quantitative PCR (RT-qPCR) Total RNA of cancer cells or tumor tissues was isolated using TRIzol ...
the131l-labeled BSA-modified CuS nanoparticles (131I-BSA@CuS) [54] The synthesized nanomaterial showed uniform dispersion, good stability and aqueous solubility, excellent photothermal properties, and long-term retention in ATC. As a therapeutic agent, it has shown remarkable efficacy in both ACT ...
The Metabolism of S35-Labeled Antithyroidal Substances in the Thyroid Gland and Other Organs of Cockerels, with Particular Reference to the Nucleic Acid Fractionsdoi:10.1530/acta.0.0180229Many investigators have given considerable thought to the mechanism of antithyroidal substances. For a review cf. ...
Following this, the cells were incubated with Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H + L) (A0423, Beyotime Biotechnology, China) for an additional hour at room temperature. Subsequently, the nuclei were subjected to staining with DAPI for a duration of 10 min, followed by ...
For double-fluorescence ISH, DIG and fluorescein signals were detected using POD-labeled antibodies and Thyramid signal amplification reactions. In sectioned ISH, specimens were dehydrated with a graded series of ethanol and embedded in polyester wax (BDH) (Nakayama et al., 2017). Embedded ...
Overview Diagram Thyroid hormone overview The thyroid hormone signaling pathway has a wide range of functions in terms of individual development, maintenance of homeostasis, cell proliferation and differentiation, and glucose metabolism. Thyroid hormones are secreted by the thyroid gland, including ...