Recently, we tested the herpes simplex virus thymidine kinase gene (tk) as both a potential positive and a negative selectable marker in T. brucei for isolating glycosome-deficient T. brucei mutants in the procyclic form and complementation studies to identify genes involved in glycosomal ...
The thymidine kinase gene may be readily isolated and mutated as described below, in order to construct nucleic acid molecules encoding a thymidine kinase gene comprising one or more mutations which substantially reduce the splicing of the gene, as compared to unmutated thymidine kinase. As utilised ...
66.A method of killing a cell, the method comprising:a) introducing into the cell a polynucleotide sequence according to any one of claims 1-26;b) allowing or initiating the cell to express the expressed thymidine kinase or variant thereof; andc) contacting the cell with an agent that is ...
The use of thymidine kinase as a selective marker in transfection experiments is established using the thymidine kinase, tk, gene of herpes simplex virus (HSV- tk ). The application of tk as a negative marker changes dramatically when substrate analogs are developed that HSV- tk recognizes but ...
Czako, et al., The herpes simplex virus thymidine kinase gene as a conditional negative-selection marker gene in Arabidopsis thaliana. Plant Physiol. Mar. 1994;104(3):1067-1071. Dahle, et al., Gap junctional intercellular communication is not a major mediator in the bystander effect in photo...
A retrovirus encoding for the herpes simplex virus tip-1 (HSV) thymidine kinase (tk) gene was generated in which expression of tk is under control of the myeloproliferative sarcoma virus (MPSV) promoter/enhancer. We used human mutated dihydrofolate reductase (DHFR) cDNA as a selectable marker....
The present invention provides isolated nucleic acid molecules encoding a Herpesviridae thymidine kinase enzyme comprising one or more mutations, at least one of the mutations encod
Additionally, the unique characteristic of CCV containing two copies of the TK gene did act prevent the use of this gene as a selectable site of homologous recombination.Huang Ge ZhangLarry A. HansonVirologyZhang H G,Hanson L A.Deletion of thymidine kinase gene attenuates channel catfish ...
Additionally, the unique characteristic of CCV containing two copies of the TK gene did act prevent the use of this gene as a selectable site of homologous recombination.HuangGeZhangandLarryA.HansonSDOSVirologyZhang H G,Hanson L A.Deletion of thymidine kinase gene attenuates channel catfish ...