Figure 1. Thy1 transgenic mice expressing GCaMP6s or GCaMP6f. a. Schematic of the transgene cassettes used to generate GP4.x (top) and GP5.x (bottom) lines. WPRE =Woodchuck hepatitis virus post-transcriptional regulatory element, pA=poly-adenylation tail. b. Wide-field images of ...
Place cellcortisolCitalopramcalcium imagingminiature microscopesGCaMP6fTransgenic miceThe hippocampus, a structure essential for spatial navigation and memory undergoes anatomical and functional changes during chronic stress. Here, we investigate the effects of chronic stress on the ability of place cells to...
Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines ...
Furthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice.HodDanaTsai-WenChenAmyHuBrenda CShieldsCaiyingGuoPloS oneDana, H., Chen, T.W., Hu, A., Shields, B.C., Guo, C., Looger, L.L., Kim, D.S., and Svoboda,...
Thy1 transgenic mice expressing GCaMP6s or GCaMP6f.Hod, DanaTsaiWen, ChenAmy, HuBrenda, C. ShieldsCaiying, GuoLoren, L. LoogerDouglas, S. KimKarel, Svoboda
BACKGROUND :The genetically encoded calcium (Ca 2+ ) sensor GCaMP6 has been widely used for imaging Ca 2+ transients in neuronal somata, dendrites, and synapses. NEW METHOD :Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and ...