其中Thy1阳性标记的细胞表现出对恐惧抑制或者被称为“恐惧-关(Fear-Off)”神经元的特性[1]。在Thy1-Cre鼠BLA注射兴奋性化学遗传学病毒AAV-DIO-Gs-DREADD-IRES-mCherry,同时以AAV-CaMKII-eYFP作对照,可见Gs- DREADD的表达局限在BLA,但是对照病毒的荧光则广泛地分布在整个杏仁核。在动物进行恐惧消退和消退维持实验...
其中Thy1阳性标记的细胞表现出对恐惧抑制或者被称为“恐惧-关(Fear-Off)”神经元的特性[1]。在Thy1-Cre鼠BLA注射兴奋性化学遗传学病毒AAV-DIO-Gs-DREADD-IRES-mCherry,同时以AAV-CaMKII-eYFP作对照,可见Gs- DREADD的表达局限在BLA,但是对照病毒的荧光则广泛地分布在整个杏仁核。在动物进行恐惧消退和消退维持实验...
Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice Brain Res., 1345 (2010), pp. 59-72 Google Scholar [15] J. Y. Wang, S. Zhang, J. Mori Axonal growth and guidance defects in Frizzled3 knock-Out mice: a comparison of...
2,3. To examine the lineage potential of Thy1+cells, we crossed inducibleThy1-CreERT2(Slick-H) mice. In these animals, back-to-backThy1promoters were used in order to drive both CreERT2 and to specifically label neurons with EYFP. As expected, we detected high levels of EYFP in the ...
HH-CAS-186 pGL3-U6-sgRNA-EYFP HH-CAS-185 pGL3-U6-sgRNA-EGFP(Plasmid #107721) HH-CAS-183 pGL3-U6-sgRNA-EBFP HH-CAS-182 pLV-U6-NLS-CasRx HH-CAS-181 pLV-U6-NLS-CasRx-P2A-Puro HH-CAS-180 pLV-U6-CasRxN-EGFP-P2A-puro HH-CAS-179 pLV-U6-CasRxN-EGFP-P2A-BSD ...