Multivariate analysis of RNA levels from postmortem human brains as measured by three different methods of RT-PCR. Stanley Neuropathology Consortium. J Neurosci Methods. 1997;77:83-92.Johnston NL, Cervenak J, Shore AD, Torrey EF, Yolken RH (1997) Multivariate analysis of RNA levels from post-...
One of the easiest ways to extend imaging duration in fluorescence microscopy is to lower the illumination intensity. This approach is ultimately limited by signal-to-noise ratio (SNR) in the raw data, a particularly important constraint in conventional 3D SIM reconstruction, which is highly suscept...
RNA:This is a form of nucleic acid that is transcribed from the DNA sequence in cells. It performs most of the cellular work but is often degraded and produced again in the cell. In prokaryotes, the mRNA can immediately be transcribed in the ribosome, but in eukaryotes,...
It is actively involved in cellular protein synthesis and carries genetic information in viruses (similar to the role of DNA is most other organisms). RNA's four nitrogenous bases are similar to DNA - adenine, cytosine, guanine - but it uses uracil in place of thymine.Answer and Explana...
The scaffolds self-assembled by nucleic acids (viz. DNA and RNA) or peptides appear as a budding branch. Unlike common hydrogels/scaffolds made from polysaccharides and proteins, they are active in immunity by self-participation which might be applicable as cancer vaccines [146,147]. Such 3D ...
RNA concentration and purity was evaluated via an ND1000 spectrophotometer (Nanodrop Technologies, USA); cDNA was synthetized using iScript (BIO-RAD, ™Veenendaal, The Netherlands) according to manufacturer's protocol. Quantitative polymerase chain reaction (qPCR) was performed on the obtained ...
Following first-strand cDNA synthesis, second strand was made by nicking the RNA templates and then extending and copying the cDNA strand using DNA polymerase I17,45,46. The reaction was terminated with EDTA and this as well as all the following reactions were performed using an optimized ...
As a consequence, it is essential to develop new food technologies and alternate ways to address the aforementioned concerns15. Cultured meat (CM) not only promotes environmental aims16 but also has the potential to produce “clean meat“17,18. CM, a disruptive future food technology that ...
What is the function of the DNA polymerase? Is it for DNA replication or the RNA replication? What is the function of DNA ligase in DNA replication? Answer the following questions regarding PCR reactions: \\ a. What do dNTPs provide during DNA replication?...
While the majority of RNA viruses bearing L-domains are known to hijack the ESCRT pathway to complete the budding process, the budding of large and complex enveloped DNA viruses, especially iridoviruses, has been rarely investigated. In the present study, we use the tiger frog virus (TFV) ...