Actin cytoskeleton, one of three types of cytoskeleton, participates in various cell events, such as migration, differentiation, links to integrins mediating cell matrix interaction and involved in mechanical signal transduction, by reorganizing the actin structure. Cells behave differently from three-...
Three types of elements that form the cytoskeleton Microfilaments, intermediate, microtubules Which element in the cytoskeleton is the smallest? Microfilaments Which element in the cytoskeleton is the largest? Microtubules What are centrioles?
PSD-95 is a postsynaptic scaffold protein that tethers NMDA receptors to the cytoskeleton. Cdk5 catalyzes PSD-95 at three residues near the N-terminal domain, which causes a reduction in co-clustering of PSD-95 and neuronal ion channels [67]. Conversely, inhibition of Cdk5 function enhances ...
by SPI-1 that collectively induce the host cell to rearrange its actincytoskeletonand induce a massive membrane ruffle that serves to engulf the bacteria. The effector molecules of aYersiniapathogenicityisland have the exact opposite role as those from SPI-1: namely, they inhibitphagocytosisof the...
cytoskeleton. Cdk5 catalyzes PSD-95 at three residues near the N-terminal domain, which causes a reduction in co-clustering of PSD-95 and neuronal ion channels [67]. Conversely, inhibition of Cdk5 function enhances PSD-95 clustering [67]. Cdk5-mediated phosphorylation of PSD-95 increases the ...
Cytoskeletons with three-dimensional architecture were isolated from cultured normal rat kidney cells. The preparation procedure consisted of Triton-demembranization of suspended cells followed by differential and sucrose density gradient centrifugation. By using higher (0.5%) and lower (0.1%) Triton conc...
Host cells injected with Salmonella effector proteins of type III secretion system (T3SS) triggering a series of responses in the host cells, including the reorganization of the cytoskeleton, inflammatory responses, and cell death (Helaine et al., 2014). In this study, the "Salmonella infection"...
These include chitin fibrils in arthropod cuticle1, the spiralling fibrillar structure in wood cells2 or bone lamellae3 and actin fibrils in the cytoskeleton4. These networks are usually far from spatially homogeneous, but vary at multiple length scales (e.g. at the nanometre and micrometre scale...
Actin can be stained in living and fixed cells to determine and follow the structure and function of the cytoskeleton. Here, the aim of the phalloidin staining was to confirm and visualize whether the living cells can aggregate and form spheroids. Regarding cell viability, the cells within the ...
Microtubules were stained with mouse monoclonal 13-tubulin antibodies (ATN01, Cytoskeleton) for 30 min and then goat anti-mouse secondary antibodies for 30 min. The secondary antibodies were labeled with amine-reactive Alexa 647 and Cy3 and the labeling stoichiometry was characterized to be −4.0...